Mouse anti-Human Sarcomeric Actinin Alpha 2/ACTN2 Monoclonal Antibody | anti-ACTN2 antibody

Sarcomeric Actinin Alpha 2/ACTN2

Cat. Num. AAA23942

• Gene Names: ACTN2; CMH23; CMD1AA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Sarcomeric Actinin Alpha 2/ACTN2, Antibody; Sarcomeric Actinin Alpha 2/ACTN2; Actin binding protein; Alpha actinin skeletal muscle, isoform 2; CMD1AA; F-actin cross-linking protein; anti-ACTN2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: ACTN2/3293
• Specificity: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 686

• Applicable Applications for anti-ACTN2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Skeletal or Cardiac Muscle.
• Immunogen: A recombinant fragment (aa557-692) of human ACTN2 protein (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 1q43
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3293). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD€™s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD€™s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

WB (Western Blot)

(Western Blot of human skeletal muscle tissue lysates using Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3293).)

SDS-PAGE

(SDS-PAGE Analysis Purified ACTN2 Mouse Monoclonal Antibody (ACTN2/3293). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human cardiac muscle stained with Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3293).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Skeletal Muscle stained with Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3293))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Skeletal Muscle stained with Sarcomeric Actinin Alpha 2 Mouse Monoclonal Antibody (ACTN2/3293).)

References

Baris, O., et al. Oncogene 24 (April 4, 2005): 4155.

NCBI and Uniprot Product Information

• NCBI GI #: 507588250
• NCBI GeneID: 88
• NCBI Accession #: NP_001265273.1
• NCBI GenBank Nucleotide #: NP_001265273.1
• UniProt Accession #: P35609
• Molecular Weight: 103kDa
• NCBI Official Full Name: alpha-actinin-2 isoform 3
• NCBI Official Synonym Full Names: actinin alpha 2
• NCBI Official Symbol: ACTN2
• NCBI Official Synonym Symbols: CMH23; CMD1AA
• NCBI Protein Information: alpha-actinin-2
• UniProt Protein Name: Alpha-actinin-2
• UniProt Gene Name: ACTN2
• UniProt Entry Name: ACTN2_HUMAN