IP (Immunoprecipitation)
(SERPINB1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-SERPINB1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SERPINB1 mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)
Mouse anti-Human SERPINB1 Monoclonal Antibody | anti-SerpinB1 antibody
Anti-SERPINB1 Antibody, Mouse Monoclonal
Gene Names
SERPINB1; EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Synonyms
SERPINB1, Antibody; Anti-SERPINB1 Antibody, Mouse Monoclonal; SerpinB1/ELANH2 Antibody, Mouse MAb; serpin peptidase inhibitor, clade B (ovalbumin), member 1; Anti-EI Antibody; Anti-ELANH2 Antibody; Anti-HEL-S-27 Antibody; Anti-HEL57 Antibody; Anti-LEI Antibody; Anti-M/NEI Antibody; Anti-MNEI Antibody; Anti-PI-2 Antibody; Anti-PI2 Antibody; anti-SerpinB1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
05
Specificity
Human SerpinB1/ELANH2
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SerpinB1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Recombinant Human SerpinB1/ELANH2 protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SerpinB1/ELANH2 (rh SerpinB1/ELANH2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(SERPINB1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-SERPINB1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SERPINB1 mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)
IP (Immunoprecipitation)
(SERPINB1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-SERPINB1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SERPINB1 mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 43 kDaObserved band size: 43 kDa)
WB (Western Blot)
(Anti-SERPINB1 mouse monoclonal antibody at 1:500 dilutionLane A: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:43 kDa)
WB (Western Blot)
(Anti-SERPINB1 mouse monoclonal antibody at 1:500 dilutionLane A: NCI-H460 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:43 kDa)
Related Product Information for anti-SerpinB1 antibody
SerpinB1 is an endogenous inhibitor of serine proteases recognized for its anti-inflammatory and host-protective properties. Serum SerpinB1 levels are elevated in patients with type 2 diabetes compared with that in healthy subjects and are negatively correlated with serum LDL-C. serpinB1 as an endogenous protein that can potentially be harnessed to enhance functional beta cell mass in patients with diabetes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,742 Da
NCBI Official Full Name
leukocyte elastase inhibitor
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 1
NCBI Official Symbol
SERPINB1
NCBI Official Synonym Symbols
EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2
NCBI Protein Information
leukocyte elastase inhibitor; peptidase inhibitor 2; epididymis luminal protein 57; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1
UniProt Protein Name
Leukocyte elastase inhibitor
UniProt Gene Name
SERPINB1
UniProt Synonym Gene Names
ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2
UniProt Entry Name
ILEU_HUMAN
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Product Notes
The SerpinB1 serpinb1 (Catalog #AAA255595) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-SERPINB1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINB1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SerpinB1 serpinb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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