Mouse anti-Human SHMT1 Monoclonal Antibody | anti-SHMT1 antibody

SHMT1 (Serine Hydroxymethyltransferase, Cytosolic, SHMT, Serine Methylase, Glycine Hydroxymethyltransferase)

Cat. Num. AAA24103

• Gene Names: SHMT1; SHMT; CSHMT
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SHMT1; Monoclonal Antibody; SHMT1 (Serine Hydroxymethyltransferase; Cytosolic; SHMT; Serine Methylase; Glycine Hydroxymethyltransferase); Anti -SHMT1 (Serine Hydroxymethyltransferase; anti-SHMT1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4F9
• Specificity: Recognizes human SHMT1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-SHMT1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Recombinant corresponding to aa374-483 from human SHMT1 with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Application Data

(Detection limit for recombinant GST tagged SHMT1 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot)

(Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody.)

WB (Western Blot)

(SHMT1 monoclonal antibody Western Blot analysis of SHMT1 expression in HeLa.)

IHC (Immunohistochemistry)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-SHMT1 antibody

This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Product Categories/Family for anti-SHMT1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 22547189
• NCBI GeneID: 6470
• NCBI Accession #: NP_683718.1
• NCBI GenBank Nucleotide #: NM_148918.2
• UniProt Accession #: P34896; Q96HY0; Q9UMD1; Q9UMD2; D3DXD0
• Molecular Weight: 53,083 Da
• NCBI Official Full Name: serine hydroxymethyltransferase, cytosolic isoform 2
• NCBI Official Synonym Full Names: serine hydroxymethyltransferase 1 (soluble)
• NCBI Official Symbol: SHMT1
• NCBI Official Synonym Symbols: SHMT; CSHMT
• NCBI Protein Information: serine hydroxymethyltransferase, cytosolic; serine methylase; glycine hydroxymethyltransferase; cytoplasmic serine hydroxymethyltransferase
• UniProt Protein Name: Serine hydroxymethyltransferase, cytosolic
• UniProt Gene Name: SHMT1
• UniProt Synonym Gene Names: SHMT
• UniProt Entry Name: GLYC_HUMAN