IF (Immunofluorescence)
(ICC/IF analysis of SHMT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SHMT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human SHMT1 Monoclonal Antibody | anti-SHMT1 antibody
SHMT1 antibody
Gene Names
SHMT1; SHMT; CSHMT
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
SHMT1, Antibody; SHMT1 antibody; Serine hydroxymethyltransferase 1; CSHMT; SHMT; anti-SHMT1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT26E5
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
483
Applicable Applications for anti-SHMT1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human SHMT1 (1-483aa) purified from E. coli
Clone Note
Anti-human SHMT1 mAb, clone AT26E5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SHMT1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of SHMT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SHMT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of SHMT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SHMT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SHMT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate Lane 2.: HeLa cell lysate Lane 3.: NIH-3T3 cell lysate Lane 4.: LnCaP cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SHMT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate Lane 2.: HeLa cell lysate Lane 3.: NIH-3T3 cell lysate Lane 4.: LnCaP cell lysate)
Related Product Information for anti-SHMT1 antibody
Serine hydroxymethyltransferase 1, also known as SHMT1, belongs to the SHMT family. This protein is cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5 10-methylene tetrahydrofolate. Also, SHMT1 preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate and inhibits.
Product Categories/Family for anti-SHMT1 antibody
References
Garrow TA., et al. (1993) J Biol Chem. 268 (16): 11910-6.; Perry C., et al. (2005) J Biol Chem. 280 (1): 396-400. ; Pinthong C., et al. (2014) FEBS J. 281 (11): 2570-83.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,672 Da
NCBI Official Full Name
Serine hydroxymethyltransferase 1 (soluble)
NCBI Official Synonym Full Names
serine hydroxymethyltransferase 1
NCBI Official Symbol
SHMT1
NCBI Official Synonym Symbols
SHMT; CSHMT
NCBI Protein Information
serine hydroxymethyltransferase, cytosolic
UniProt Protein Name
Serine hydroxymethyltransferase, cytosolic
UniProt Gene Name
SHMT1
UniProt Synonym Gene Names
SHMT
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Product Notes
The SHMT1 shmt1 (Catalog #AAA48224) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SHMT1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SHMT1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SHMT1 shmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHMT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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