IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-SiderocalinantibodyMAB1857(AAA72494). ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cells,permeabilizedwith0.15%Triton,onShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofMAB1857(AAA72494)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72494,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
Siderocalin Recombinant Antibody | Lcn2 recombinant antibody
Anti-Siderocalin [MAB1857], Rat IgG2a, kappa
Gene Names
Lcn2; 24p3; Sip24; AW212229
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Protein A affinity purified
Synonyms
Siderocalin, Recombinant Antibody; Anti-Siderocalin [MAB1857], Rat IgG2a, kappa; Siderocalin; Anti-Siderocalin [MAB1857]; 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; 228418; Lcn2 recombinant antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
MAB1857
Specificity
Detects recombinant mouse (rm)Lipocalin-2 and does not cross-react with rhLipocalin-1 or rhLipocalin?2 by ELISA or WB.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for Lcn2 recombinant antibody
ELISA, WB (Western Blot)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-SiderocalinantibodyMAB1857(AAA72494). ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cells,permeabilizedwith0.15%Triton,onShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofMAB1857(AAA72494)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72494,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofRAW264.7cellswithanti-SiderocalinantibodyMAB1857(AAA72494). ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cells,permeabilizedwith0.15%Triton,onShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofMAB1857(AAA72494)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingcytoplasmicstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72494,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot)
(Western Blotusinganti-SiderocalinantibodyMAB1857(AAA72494). Mousespleentissuelysate(35ugproteininRIPAbuffer)wasresolvedviaSDS-PAGE,andthesubsequentblotwasprobedwiththechimericrabbitversionofMAB1857(AAA72494)at0.1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hourwasused,andproteinsweredetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-SiderocalinantibodyMAB1857(AAA72494). Mousespleentissuelysate(35ugproteininRIPAbuffer)wasresolvedviaSDS-PAGE,andthesubsequentblotwasprobedwiththechimericrabbitversionofMAB1857(AAA72494)at0.1ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hourwasused,andproteinsweredetectedbychemiluminescence.)
Related Product Information for Lcn2 recombinant antibody
Recombinant mouse Lipocalin-2/NGAL - Gln21-Asn200.
References
Nairz M et al. 2008 Interferon-gamma limits the availability of iron for intramacrophage Salmonella typhimurium Eur J Immunol. 2008 Jul;38(7):1923-36. doi: 10.1002/eji.200738056. N/A18581323
Note on publication:
Describes how this antibody was used to detect siderocalin via immunoblotting.
Note on publication:
Describes how this antibody was used to detect siderocalin via immunoblotting.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,875 Da
NCBI Official Full Name
neutrophil gelatinase-associated lipocalin
NCBI Official Synonym Full Names
lipocalin 2
NCBI Official Symbol
Lcn2
NCBI Official Synonym Symbols
24p3; Sip24; AW212229
NCBI Protein Information
neutrophil gelatinase-associated lipocalin
UniProt Protein Name
Neutrophil gelatinase-associated lipocalin
UniProt Gene Name
Lcn2
UniProt Synonym Gene Names
NGAL
UniProt Entry Name
NGAL_MOUSE
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Product Notes
The Lcn2 lcn2 (Catalog #AAA72494) is a Recombinant Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Siderocalin [MAB1857], Rat IgG2a, kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Siderocalin can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Lcn2 lcn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Siderocalin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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