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WB (Western Blot)
(Detection of human SIRP alpha by western blot. Samples: Whole cell lysate (50 ug) from Malme-3M, HeLa, 786-O, HEK293T, U2OS, OVCAR-4, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin .)
SIRP alpha Recombinant Antibody | SIRPalpha recombinant antibody
Rabbit anti-SIRP alpha Recombinant Monoclonal Antibody [BLR049F]
Gene Names
SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
SIRP alpha, Recombinant Antibody; Rabbit anti-SIRP alpha Recombinant Monoclonal Antibody [BLR049F]; Sirp-alpha-1; SHP substrate 1; SHPS1; SHPS-1; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; SIRP; myd-1 antigen; Sirp-alpha-2; Sirp-alpha-3; tyrosine phosphatase SHP substrate 1; tyrosine-protein phosphatase non-receptor type substrate 1; Signal-regulatory protein alpha-3; P84; MYD-1; MFR; macrophage fusion receptor; inhibitory receptor SHPS-1; CD172A; CD172 antigen-like family member A; CD antigen CD172a; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; BIT; PTPNS1; Tyrosine-protein phosphatase non-receptor type substrate 1; signal regulatory protein alpha; SIRPalpha recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR049F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for SIRPalpha recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 31-368 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human SIRP alpha by western blot. Samples: Whole cell lysate (50 ug) from Malme-3M, HeLa, 786-O, HEK293T, U2OS, OVCAR-4, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin .)
WB (Western Blot)
(Detection of human SIRP alpha by western blot. Samples: Whole cell lysate (50 ug) from Malme-3M, HeLa, 786-O, HEK293T, U2OS, OVCAR-4, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin .)
IP (Immunoprecipitation)
(Detection of human SIRP alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-MEL-28 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used for IP at 20 ul per reaction. SIRP alpha was also immunoprecipitated by rabbit anti-SIRP alpha recombinant monoclonal antibody [BL-565-1D6]. For blotting immunoprecipitated SIRP alpha, AAA213547 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human SIRP alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-MEL-28 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used for IP at 20 ul per reaction. SIRP alpha was also immunoprecipitated by rabbit anti-SIRP alpha recombinant monoclonal antibody [BL-565-1D6]. For blotting immunoprecipitated SIRP alpha, AAA213547 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohiostchemistry)
(Detection of human SIRP alpha by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human SIRP alpha by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human SIRP alpha by immunocytochemistry. Sample: FFPE section of Malme-3M cells. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human SIRP alpha by immunocytochemistry. Sample: FFPE section of Malme-3M cells. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (AAA213547 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for SIRPalpha recombinant antibody
SIRP alpha is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRP alpha is a cell surface receptor for CD47 and CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. [taken from NCBI Entrez Gene (Gene ID: 140885)].
Product Categories/Family for SIRPalpha recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,967 Da
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type substrate 1
NCBI Official Synonym Full Names
signal-regulatory protein alpha
NCBI Official Symbol
SIRPA
NCBI Official Synonym Symbols
BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; OTTHUMP00000030001; OTTHUMP00000159968; OTTHUMP00000159969; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP sub
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type substrate 1
UniProt Gene Name
SIRPA
UniProt Synonym Gene Names
BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; Sirp-alpha-2
UniProt Entry Name
SHPS1_HUMAN
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Product Notes
The SIRPalpha sirpa (Catalog #AAA213547) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SIRP alpha Recombinant Monoclonal Antibody [BLR049F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIRP alpha can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SIRPalpha sirpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRP alpha, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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