IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SIRT5 protein using SIRT5 antibody)
Mouse SIRT5 Monoclonal Antibody | anti-SIRT5 antibody
SIRT5 antibody
Gene Names
SIRT5; SIR2L5
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
SIRT5 antibody was purified by affinity chromatography.
Synonyms
SIRT5, Antibody; SIRT5 antibody; Monoclonal SIRT5; Anti-SIRT5; Sirtuin; Silent Mating Type Information Regulation 2 homolog 5; SIR2L5; SIRT5; SIRT 5; SIRT-5; anti-SIRT5 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2A11
Purity/Purification
SIRT5 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
310
Applicable Applications for anti-SIRT5 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
SIRT5 is included in class III of the sirtuin family which ischaracterized by a sirtuin core domain. Human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing. and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
Cross-Reactivity
Human
Immunogen
SIRT5 antibody was raised in mouse using a full length recombinant protein of human SIRT5 (NP_036373) produced in HEK293 cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SIRT5 protein using SIRT5 antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SIRT5 protein using SIRT5 antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of SIRT5 protein in paraffin embedded Human Kidney tissue using SIRT5 antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of SIRT5 protein in paraffin embedded Human Kidney tissue using SIRT5 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SIRT5 protein (Right) or empty vector (Left) detected with SIRT5 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SIRT5 protein (Right) or empty vector (Left) detected with SIRT5 antibody)
Related Product Information for anti-SIRT5 antibody
Mouse monoclonal SIRT5 antibody
Product Categories/Family for anti-SIRT5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,689 Da
NCBI Official Full Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial isoform 1
NCBI Official Synonym Full Names
sirtuin 5
NCBI Official Symbol
SIRT5
NCBI Official Synonym Symbols
SIR2L5
NCBI Protein Information
NAD-dependent protein deacylase sirtuin-5, mitochondrial
UniProt Protein Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial
UniProt Gene Name
SIRT5
UniProt Entry Name
SIR5_HUMAN
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Product Notes
The SIRT5 sirt5 (Catalog #AAA106684) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SIRT5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the SIRT5 sirt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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