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product-image-AAA213524_WB10.jpg WB (Western Blot) (Detection of mouse Skeletal Troponin I by western blot. Samples: Whole tissue lysate (50 ug) from mouse liver and skeletal muscle. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody [FI-23] (AAA213524 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 1 second.)

Mouse anti-Human, Mouse Skeletal Troponin I Monoclonal Antibody | anti-TNNI2 antibody

Mouse anti-Skeletal Troponin I Monoclonal Antibody [FI-23]

Gene Names
TNNI2; DA2B; FSSV; fsTnI; AMCD2B
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
Skeletal Troponin I, Antibody; Mouse anti-Skeletal Troponin I Monoclonal Antibody [FI-23]; DA2B; troponin I, skeletal, fast; troponin I, fast-twitch skeletal muscle isoform; troponin I, fast-twitch isoform; troponin I, fast skeletal muscle; troponin I type 2 (skeletal, fast); troponin I fast twitch 2; FSSV; AMCD2B; fsTnI; Troponin I, fast skeletal muscle; troponin I2, fast skeletal type; anti-TNNI2 antibody
Ordering
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
FI-23
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TNNI2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Human Skeletal Troponin I
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse Skeletal Troponin I by western blot. Samples: Whole tissue lysate (50 ug) from mouse liver and skeletal muscle. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody [FI-23] (AAA213524 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 1 second.)

product-image-AAA213524_WB10.jpg WB (Western Blot) (Detection of mouse Skeletal Troponin I by western blot. Samples: Whole tissue lysate (50 ug) from mouse liver and skeletal muscle. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody [FI-23] (AAA213524 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 1 second.)

WB (Western Blot)

(Detection of human Skeletal Troponin I by western blot. Samples: Whole cell lysate (50 ug) from human skeletal muscle tissue lysate and HEK293T cell lysate. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody [FI-23] (AAA213524 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)

product-image-AAA213524_WB11.jpg WB (Western Blot) (Detection of human Skeletal Troponin I by western blot. Samples: Whole cell lysate (50 ug) from human skeletal muscle tissue lysate and HEK293T cell lysate. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody [FI-23] (AAA213524 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohiostchemistry)

(Detection of human Skeletal Troponin I by immunohistochemistry. Sample: FFPE section of human skeletal muscle. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody [FI-23] (AAA213524 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)

product-image-AAA213524_IHC13.jpg IHC (Immunohiostchemistry) (Detection of human Skeletal Troponin I by immunohistochemistry. Sample: FFPE section of human skeletal muscle. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody [FI-23] (AAA213524 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)

IHC (Immunohistochemistry)

(Detection of mouse Skeletal Troponin I by immunohistochemistry. Sample: FFPE section of mouse skeletal muscle. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody [FI-23] (AAA213524 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)

product-image-AAA213524_IHC15.jpg IHC (Immunohistochemistry) (Detection of mouse Skeletal Troponin I by immunohistochemistry. Sample: FFPE section of mouse skeletal muscle. Antibody: Mouse anti-Skeletal Troponin I monoclonal antibody [FI-23] (AAA213524 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)
Related Product Information for anti-TNNI2 antibody
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. The Troponin I subfamily contains three genes: Troponin I-skeletal-fast-twitch, Troponin I-skeletal-slow-twitch, and Troponin I-cardiac. Troponin I-cardiac is exclusively expressed in cardiac muscle tissues. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P19429].
Product Categories/Family for anti-TNNI2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,355 Da
NCBI Official Full Name
troponin I, fast skeletal muscle isoform 1
NCBI Official Synonym Full Names
troponin I type 2 (skeletal, fast)
NCBI Official Symbol
TNNI2
NCBI Official Synonym Symbols
DA2B; FSSV; fsTnI; AMCD2B
NCBI Protein Information
troponin I, fast skeletal muscle; troponin I fast twitch 2; troponin I, skeletal, fast; troponin I, fast-twitch isoform; troponin I, fast-twitch skeletal muscle isoform
UniProt Protein Name
Troponin I, fast skeletal muscle
UniProt Gene Name
TNNI2
UniProt Entry Name
TNNI2_HUMAN

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Product Notes

The TNNI2 tnni2 (Catalog #AAA213524) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Skeletal Troponin I Monoclonal Antibody [FI-23] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Skeletal Troponin I can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TNNI2 tnni2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Skeletal Troponin I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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