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product-image-AAA300739_AD11.jpg Application Data (Jurkat cells stained with SMAD5 (red, 1:100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG)

Mouse SMAD5 Monoclonal Antibody | anti-SMAD5 antibody

SMAD5(C-term) Monoclonal Antibody

Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human, Porcine, Rat, Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Western Blot
Purity
Purified by antigen-affinity chromatography.
Synonyms
SMAD5, Antibody; SMAD5(C-term) Monoclonal Antibody; mothers against decapentaplegic homolog 5 SMA-and MAD-related protein 5 SMAD, mothers against DPP homolog 5 MAD, mothers against decapentaplegic homolog 5 mothers against decapentaplegic, drosophila, homolog of, 5; anti-SMAD5 antibody
Ordering
Host
Mouse
Reactivity
Human, Porcine, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
Purified by antigen-affinity chromatography.
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Sequence Length
465
Applicable Applications for anti-SMAD5 antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen Type
Recombinant protein
Immunogen Description
Recombinant fragment, corresponding to amino acids 239aa-372aa of human SMAD5 (C-terminus).
Target Name
SMAD5
Preparation and Storage
store at -20 degree C

Application Data

(Jurkat cells stained with SMAD5 (red, 1:100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG)

product-image-AAA300739_AD11.jpg Application Data (Jurkat cells stained with SMAD5 (red, 1:100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG)

Application Data

(HeLa cells using anti- SMAD5 (C-terminus) antibody diluted 1:150; Flow Cytometry analysis Jurkat cells stained with SMAD5 (red, 1:100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal m)

product-image-AAA300739_AD13.jpg Application Data (HeLa cells using anti- SMAD5 (C-terminus) antibody diluted 1:150; Flow Cytometry analysis Jurkat cells stained with SMAD5 (red, 1:100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal m)

Application Data

(1:1000 dilution of this antibody detected SMAD5 on 40ug of cell lysates)

product-image-AAA300739_AD15.jpg Application Data (1:1000 dilution of this antibody detected SMAD5 on 40ug of cell lysates)
Product Categories/Family for anti-SMAD5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,258 Da
NCBI Official Full Name
mothers against decapentaplegic homolog 5
NCBI Official Synonym Full Names
SMAD family member 5
NCBI Official Symbol
SMAD5
NCBI Official Synonym Symbols
DWFC; JV5-1; MADH5
NCBI Protein Information
mothers against decapentaplegic homolog 5
UniProt Protein Name
Mothers against decapentaplegic homolog 5
UniProt Gene Name
SMAD5
UniProt Synonym Gene Names
MADH5; MAD homolog 5; Mothers against DPP homolog 5; SMAD 5; Smad5; hSmad5
UniProt Entry Name
SMAD5_HUMAN

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Product Notes

The SMAD5 smad5 (Catalog #AAA300739) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SMAD5(C-term) Monoclonal Antibody reacts with Human, Porcine, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD5 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SMAD5 smad5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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