Rabbit anti-Human SMC1-S957 Monoclonal Antibody | anti-SMC1A antibody

Phospho-SMC1-S957 Rabbit mAb

Cat. Num. AAA28615

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity purification
• Synonyms: SMC1-S957, Antibody; Phospho-SMC1-S957 Rabbit mAb; SMC1; SMCB; CDLS2; DEE85; SB1.8; EIEE85; SMC1L1; DXS423E; SMC1alpha; Phospho-SMC1-S957; anti-SMC1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
• Sequence: GSSQG

• Applicable Applications for anti-SMC1A antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; ELISA: Recommended starting concentration is 1ug/mL.; Please optimize the concentration based on your specific assay requirements.
• Cross Reactivity: Human, Mouse, Rat
• Immunogen: A synthetic phosphorylated peptide around S957 of human SMC1 (Q14683).
• Modification: Phospho S957
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Phospho-SMC1-S957 Rabbit mAb (AAA28615) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Phospho-SMC1-S957 Rabbit mAb (AAA28615) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-SMC1-S957 Rabbit mAb (AAA28615) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Phospho-SMC1-S957 Rabbit mAb (AAA28615) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Phospho-SMC1-S957 Rabbit mAb (AAA28615) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-SMC1-S957 Rabbit mAb (AAA28615) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)

WB (Western Blot)

(Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SMC1-S957 Rabbit mAb (AAA28615) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.)

WB (Western Blot)

(Western blot analysis of lysates from 293T cells, using Phospho-SMC1-S957 Rabbit mAb (AAA28615) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Related Product Information for anti-SMC1A antibody

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Categories/Family for anti-SMC1A antibody

Epigenetics Nuclear Signaling; Protein phosphorylation; ATM Signaling Pathway; Cell Biology Developmental Biology; Apoptosis; Cell Cycle; Phosphorylated Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 30581135
• NCBI GeneID: 8243
• NCBI Accession #: NP_006297.2
• NCBI GenBank Nucleotide #: NM_006306.2
• UniProt Accession #: Q14683; O14995; Q16351; Q2M228
• Molecular Weight: Calculated MW: 143kDa; Observed MW: 160kDa
• UniProt Protein Name: Structural maintenance of chromosomes protein 1A
• UniProt Gene Name: SMC1A
• UniProt Synonym Gene Names: DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; SMC protein 1A; SMC-1-alpha; SMC-1A
• UniProt Entry Name: SMC1A_HUMAN