IHC (Immunohistochemistry)
(IHC staining of human cerebellar neurons using AAA14700.)
Mouse SNAP 25, 26-27kD Monoclonal Antibody
SNAP 25, 26-27kD (Synaptosomal-associated Protein)
Reactivity
Rat, Hamster Gerbil and Porcine
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified by Protein A affinity chromatography from tissue culture supernatent
Synonyms
SNAP 25, 26-27kD, Antibody; SNAP 25, 26-27kD (Synaptosomal-associated Protein); Anti -SNAP 25, 26-27kD (Synaptosomal-associated Protein); anti-SNAP 25, 26-27kD antibody
Host
Mouse
Reactivity
Rat, Hamster Gerbil and Porcine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H2886 (SP12)
Specificity
Recognizes human SNAP-25; an antigen of 26-27kD molecular weight. Will react with SNAP-25 from COS cells, but not with the fusion protein from bacterial systems.
Purity/Purification
Purified by Protein A affinity chromatography from tissue culture supernatent
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-SNAP 25, 26-27kD antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Immunohistochemistry (Paraffin): 1:2000-1:5000
Does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections.
Western Blot: 1:500-1:5000
Optimal working dilutions to be determined by researcher.
Recommended Secondary Antibodies:
IgG, H&L (AP) Pab Gt xMo
IgG, H&L (FITC) Pab Gt xMo
IgG, H&L (HRP) Pab Gt xMo
IgG,(HRP) Pab Rb xMo
IgG (PE) Pab Gt xMo
Does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections.
Western Blot: 1:500-1:5000
Optimal working dilutions to be determined by researcher.
Recommended Secondary Antibodies:
IgG, H&L (AP) Pab Gt xMo
IgG, H&L (FITC) Pab Gt xMo
IgG, H&L (HRP) Pab Gt xMo
IgG,(HRP) Pab Rb xMo
IgG (PE) Pab Gt xMo
Immunogen
Crude human synaptic immunoprecipitate.
Hybridoma
NSO myeloma cells with spleen cells from Balb/c mice.
IHC Positive Control
Brain
Grade
Afffinity Purified
Preparation and Storage
May be stored at 4 degree for short term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree. Aliquots are stable for 12 months after receipt. For maximum recovery of profuct, centrifuge the original vial after thawing and prior to removing the cap.
IHC (Immunohistochemistry)
(IHC staining of human cerebellar neurons using AAA14700.)
IHC (Immunohistochemistry)
(IHC staining of human cerebellar neurons using AAA14700.)
Related Product Information for anti-SNAP 25, 26-27kD antibody
Research studies have used this antibody to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy (1, 3, 8).
Product Categories/Family for anti-SNAP 25, 26-27kD antibody
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The SNAP 25, 26-27kD (Catalog #AAA14700) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SNAP 25, 26-27kD (Synaptosomal-associated Protein) reacts with Rat, Hamster Gerbil and Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's SNAP 25, 26-27kD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Immunohistochemistry (Paraffin): 1:2000-1:5000 Does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections. Western Blot: 1:500-1:5000 Optimal working dilutions to be determined by researcher. Recommended Secondary Antibodies: IgG, H&L (AP) Pab Gt xMo IgG, H&L (FITC) Pab Gt xMo IgG, H&L (HRP) Pab Gt xMo IgG,(HRP) Pab Rb xMo IgG (PE) Pab Gt xMo. Researchers should empirically determine the suitability of the SNAP 25, 26-27kD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNAP 25, 26-27kD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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