WB (Western Blot)
(Western blot analysis of SOX1 expression in HeLa cell lysate (AAA124499).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX1)
Rabbit SOX1 Monoclonal Antibody | anti-SOX1 antibody
Anti-SOX1 Rabbit Monoclonal Antibody
Reactivity
Predicted Reactivity: Bovine
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Affinity-chromatography
Synonyms
SOX1, Antibody; Anti-SOX1 Rabbit Monoclonal Antibody; Transcription factor SOX-1; SOX1; anti-SOX1 antibody
Host
Rabbit
Reactivity
Predicted Reactivity: Bovine
Clonality
Monoclonal
Clone Number
Monoclonal GIA-19
Purity/Purification
Affinity-chromatography
Form/Format
Liquid
Concentration
0.5-1mg/ml (varies by lot)
Sequence Length
391
Applicable Applications for anti-SOX1 antibody
WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence)
Immunogen
A synthesized peptide derived from human SOX1
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
At -20°C for one year. Avoid repeated freezing and thawing.
WB (Western Blot)
(Western blot analysis of SOX1 expression in HeLa cell lysate (AAA124499).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX1)
WB (Western Blot)
(Western blot analysis of SOX1 expression in HeLa cell lysate (AAA124499).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX1)
Related Product Information for anti-SOX1 antibody
Introduction: rGFP, also known as Green Fluorescent Protein, is a protein produced by the jellyfish (Aequorea Victoria) that produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag. rGFP is expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. Green Fluorescent Protein permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
About: SOX1 (for Sex determining region Y-box 1), located in 13q34, is a transcription factor in the Sox protein family. It means that this intronless geneencodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development andin the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with otherproteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development. SOX1 expression is restricted to neuroectodermin the tetrapod embryo. It is expressed particularly in the ventral striatum.
Description: Anti-SOX1 Rabbit Monoclonal Antibodytested for IF, IHC, ICC, WB in Human, Mouse
About: SOX1 (for Sex determining region Y-box 1), located in 13q34, is a transcription factor in the Sox protein family. It means that this intronless geneencodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development andin the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with otherproteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development. SOX1 expression is restricted to neuroectodermin the tetrapod embryo. It is expressed particularly in the ventral striatum.
Description: Anti-SOX1 Rabbit Monoclonal Antibodytested for IF, IHC, ICC, WB in Human, Mouse
Product Categories/Family for anti-SOX1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39023 MW
NCBI Official Full Name
transcription factor SOX-1
NCBI Official Synonym Full Names
SRY-box 1
NCBI Official Symbol
SOX1
NCBI Protein Information
transcription factor SOX-1
UniProt Protein Name
Transcription factor SOX-1
UniProt Gene Name
SOX1
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Product Notes
The SOX1 sox1 (Catalog #AAA124499) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SOX1 Rabbit Monoclonal Antibody reacts with Predicted Reactivity: Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's SOX1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the SOX1 sox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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