Mouse anti-Human, Mouse SOX10 Monoclonal Antibody | anti-SOX10 antibody

SOX10 (Melanoma Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62768

• Gene Names: SOX10; DOM; WS4; PCWH; WS2E; WS4C
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Synonyms: SOX10, Antibody; SOX10 (Melanoma Marker) Mouse Monoclonal Antibody; DOM; PCWH; SOX10; SRY (sex determining region Y) box 10; SRY box containing gene 10; SRY related HMG box gene 10; Waardenburg syndrome type 2E (WS2E); WS4; Waardenburg syndrome type 4C (WS4C); anti-SOX10 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b / Kappa
• Clone Number: SOX10/1074
• Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full- length human proteins. Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti- Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately-to-strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-SOX10 antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen: Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)
• Cellular Localization: Cytoplasm, Mitochondrion outer membrane, Nucleus
• Positive Control: UACC-257, UACC-62, breast carcinomas or gliomas., COLO-38 or HepG2 cells. Human melanomas
• Research Areas: Developmental Biology, Neural Stem Cells, Nuclear Marker
• Preparation and Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Non- hazardous. No MSDS required

Application Data

(Formalin-fixed, paraffin-embedded human melanoma stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074))

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteinsusing SOX10 Mouse Monoclonal Antibody (SOX10/1074) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

WB (Western Blot)

(Western blot analysis of COLO-38 cell lysate using SOX10 Mouse Monoclonal Antibody (SOX10/1074).)

Application Data

(Formalin-fixed, paraffin-embedded mouse brain stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074).)

WB (Western Blot)

(Western blot analysis of SOX10: (A) recombinant protein; (B) A375 cell lysate using SOX10 Mouse Monoclonal Antibody (SOX10/1074))

References

Mohamed A, et al. SOX10 Expression in malignant melanoma, carcinoma, and normal tissues. Appl Immunohistochem Mol Morphol. 2013; 21(6):506-10.-

NCBI and Uniprot Product Information

• NCBI GI #: 5902104
• NCBI GeneID: 6663
• NCBI Accession #: NP_008872.1
• NCBI GenBank Nucleotide #: NM_006941.3
• UniProt Accession #: P56693; Q6FHW7; B4DV62
• Molecular Weight: Mol. Weight of Antigen: 49-58kDa
• NCBI Official Full Name: transcription factor SOX-10
• NCBI Official Synonym Full Names: SRY-box 10
• NCBI Official Symbol: SOX10
• NCBI Official Synonym Symbols: DOM; WS4; PCWH; WS2E; WS4C
• NCBI Protein Information: transcription factor SOX-10
• UniProt Protein Name: Transcription factor SOX-10
• UniProt Gene Name: SOX10
• UniProt Entry Name: SOX10_HUMAN