Mouse Stat5 (Tyr-694) Monoclonal Antibody | anti-STAT5A antibody

Stat5 (Tyr-694), phospho-specific

Cat. Num. AAA71509

• Gene Names: STAT5A; MGF; STAT5
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: Stat5 (Tyr-694), Antibody; Stat5 (Tyr-694), phospho-specific; Signal transducer and activator of transcription 5A; Stat5A; anti-STAT5A antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: The antibody detects a 92 kDa* band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells treated with EGF, but does not detect this band in control cells.
• Purity/Purification: Protein A Purified
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 794

• Applicable Applications for anti-STAT5A antibody: WB (Western Blot), ELISA
• Immunogen: Clone (M148) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 694 of human Stat5A. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.
• Preparation and Storage: Store at -20 degree C. Do not aliquot. Stable for 1 year.

WB (Western Blot)

(Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7 & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8 & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).)

ICC (Immunocytochemistry)

(Immunocytochemical labeling of Stat5 in control and pervanadatetreated A431 cells. The cells were labeled with mouse monoclonal Stat5 or Stat5 (Tyr-694) (AAA71509) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.)

Related Product Information for anti-STAT5A antibody

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types.Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFN-alpha in B cells, but only Stat5alpha is phosphorylated in response to IFN-alpha in HeLa cells.

References

• Gouilleux, F. et al. (1994) EMBO J. 13:4361.
• Wakao, H. et al. (1994) EMBO J. 13:2182.
• Wakao, H. et al. (1994) EMBO J. 13:2182.
• Darnell, J.E. (1997) Science 277:1630.

NCBI and Uniprot Product Information

• NCBI GI #: 21618342
• NCBI GeneID: 6776
• NCBI Accession #: NP_003143.2
• NCBI GenBank Nucleotide #: NM_003152.3
• UniProt Accession #: P42229; Q1KLZ6; Q59GY7; Q8WWS9
• Molecular Weight: 90516 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A; OTTHUMP00000198376
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN