Mouse SUMO2 Monoclonal Antibody | anti-SUMO2 antibody

SUMO2 Antibody (C-term)

Cat. Num. AAA283546

• Gene Names: SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• Reactivity: Human, rat (Predicted Reactivity: Bovine, Chicken, Hamster, Monkey, Mouse, Pig)
• Applications: Western Blot, ELISA, Immunofluorescence
• Synonyms: SUMO2, Antibody; SUMO2 Antibody (C-term); Small ubiquitin-related modifier 2; SUMO-2; HSMT3; SMT3 homolog 2 {ECO:0000312|HGNC:HGNC:11125}; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3B; Smt3B; SUMO2 HGNC:11125); anti-SUMO2 antibody

• Host: Mouse
• Reactivity: Human, rat (Predicted Reactivity: Bovine, Chicken, Hamster, Monkey, Mouse, Pig)
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 973CT8.1.1
• Specificity: Purified His-tagged SUMO2 protein was used to produced this monoclonal antibody.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 71

• Applicable Applications for anti-SUMO2 antibody: WB (Western Blot), ELISA, IF (Immunofluorescence)
• Antigen Type: Recombinant Protein
• Crown Antibody: Yes
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(SUMO2 Antibody (C-term) western blot analysis in CEM,293,rat C6 cell line lysates (35ug/lane).This demonstrates the SUMO2 antibody detected the SUMO2 protein (arrow).)

IF (Immunofluorescence)

(Fluorescent image of Hela cells stained with SUMO2 Antibody (C-term). AAA283546 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

Related Product Information for anti-SUMO2 antibody

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.

Product Categories/Family for anti-SUMO2 antibody

Crown Antibodies; Cell Biology

References

Reverter D., et al. Structure 12:1519-1531(2004).
Xu Z., et al. Biochem. J. 386:325-330(2005).
Mannen H., et al. Biochem. Biophys. Res. Commun. 222:178-180(1996).
Lapenta V., et al. Genomics 40:362-367(1997).
Ota T., et al. Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 54792071
• NCBI GeneID: 6613
• NCBI Accession #: NP_001005849.1; NP_008868.3
• NCBI GenBank Nucleotide #: NM_001005849.1
• UniProt Accession #: P61956; P55855; Q32Q42; Q6IPZ6; Q96HK1; B2R4I2
• Molecular Weight: 10871
• NCBI Official Full Name: small ubiquitin-related modifier 2 isoform b
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 2
• NCBI Official Symbol: SUMO2
• NCBI Official Synonym Symbols: HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
• NCBI Protein Information: small ubiquitin-related modifier 2
• UniProt Protein Name: Small ubiquitin-related modifier 2
• UniProt Gene Name: SUMO2
• UniProt Synonym Gene Names: Smt3B
• UniProt Entry Name: SUMO2_HUMAN