IF (Immunofluorescence)
(Immunofluorescent analysis of SUMO2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
Mouse anti-Human, Rat SUMO2 Monoclonal Antibody | anti-SUMO2 antibody
Anti-SUMO2 Antibody
Gene Names
SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
Reactivity
Human, Rat
Applications
Immunofluorescence, Western Blot
Purity
This antibody is purified through a protein G column.
Synonyms
SUMO2, Antibody; Anti-SUMO2 Antibody; anti-SUMO2 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
Mouse IgG2b
Specificity
Recognizes endogenous levels of SUMO2 protein.
Purity/Purification
This antibody is purified through a protein G column.
Form/Format
Mouse IgG2b. Liquid in PBS, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-SUMO2 antibody
IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant fusion protein of human SUMO2. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of SUMO2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of SUMO2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Western blot analysis of SUMO2 expression in CEM (A), 293 (B), C6 (C) whole cell lysates. (Predicted band size: 10 kD; Observed band size: 17 kD))
WB (Western Blot)
(Western blot analysis of SUMO2 expression in CEM (A), 293 (B), C6 (C) whole cell lysates. (Predicted band size: 10 kD; Observed band size: 17 kD))
Related Product Information for anti-SUMO2 antibody
Mouse monoclonal antibody to SUMO2
Product Categories/Family for anti-SUMO2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,871 Da
NCBI Official Full Name
small ubiquitin-related modifier 2 isoform b
NCBI Official Synonym Full Names
small ubiquitin-like modifier 2
NCBI Official Symbol
SUMO2
NCBI Official Synonym Symbols
HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
NCBI Protein Information
small ubiquitin-related modifier 2; sentrin 2; SMT3 homolog 2; ubiquitin-like protein SMT3A; ubiquitin-like protein SMT3B; SMT3 suppressor of mif two 3 homolog 2
UniProt Protein Name
Small ubiquitin-related modifier 2
UniProt Gene Name
SUMO2
UniProt Synonym Gene Names
SMT3A; SMT3H2; SUMO-2; Smt3A
UniProt Entry Name
SUMO2_HUMAN
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Product Notes
The SUMO2 sumo2 (Catalog #AAA266055) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-SUMO2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SUMO2 can be used in a range of immunoassay formats including, but not limited to, IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the SUMO2 sumo2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUMO2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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