Mouse SWAP70 Monoclonal Antibody | anti-SWAP70 antibody

SWAP70 Mouse mAb

Cat. Num. AAA320353

• Gene Names: SWAP70; HSPC321; SWAP-70
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Immunoprecipitation, Western Blot
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Synonyms: SWAP70, Antibody; SWAP70 Mouse mAb; KIAA0640; 70kDa; AV235546; FLJ39540; HSPC321; SWA 70 protein; SWAP 70; SWAP switching B-cell complex 70kDa subunit; SWAP-70; SWAP70; Switch associated protein 70; Switch-associated protein 70; SWP70_HUMAN.; anti-SWAP70 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Monoclonal
• Specificity: This antibody detects endogenous levels of SWAP70 and does not cross-react with related proteins.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SWAP70 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Purified recombinant human SWAP70 protein fragments expressed in E coli.
• Modification: Unmodified/Total
• Domain: The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
• Function: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
• PTM: Tyrosine-phosphorylated.; Contains 1 PH domain.
• Subcellular Location: In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
• Subunit: The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70.
• Tissue Specificity: Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed; 10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed; 12925760).
• Preparation and Storage: Store at -20 degree C/1 year

IP (Immunoprecipitation)

(Immunoprecipitation analysis of Hela cell lysate using SWAP70 mouse mAb.)

WB (Western Blot)

(Western blot detection of SWAP70 in COS7.293T.C6.3T3 and Hela cell lysates and using SWAP70 mouse mAb (1:1000 diluted).Predicted band size: 70KDa.Observed band size: 70KDa.)

Product Categories/Family for anti-SWAP70 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 93102364
• NCBI GeneID: 23075
• NCBI Accession #: NP_055870.2
• NCBI GenBank Nucleotide #: NM_015055.3
• UniProt Accession #: Q9UH65; O75135; Q7LCY6; Q9P061; Q9P0Z8; D3DQV1
• Molecular Weight: 68,998 Da
• NCBI Official Full Name: switch-associated protein 70 isoform 1
• NCBI Official Synonym Full Names: SWAP switching B-cell complex 70kDa subunit
• NCBI Official Symbol: SWAP70
• NCBI Official Synonym Symbols: HSPC321; SWAP-70
• NCBI Protein Information: switch-associated protein 70
• UniProt Protein Name: Switch-associated protein 70
• UniProt Gene Name: SWAP70
• UniProt Synonym Gene Names: KIAA0640; SWAP-70
• UniProt Entry Name: SWP70_HUMAN