IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3 + CC49).)
Mouse TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Monoclonal Antibody | anti-TAG-72 antibody
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody
Reactivity
Human, Cow, Dog, Rat
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Antibody; TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody; CA72.4, Tumor associated glycoprotein 72; anti-TAG-72 antibody
Host
Mouse
Reactivity
Human, Cow, Dog, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
B72.3 + CC49
Specificity
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the apocrine carcinoma.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-TAG-72 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Cytoplasmic and Cell Surface
Immunogen
Membrane-enriched fraction of a human breast carcinoma liver metastasis (B72.3); Purified TAG-72 protein (CC49)
Positive Control
Jurkat cells, Breast or lung carcinoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3 + CC49).)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3 + CC49).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3 + CC49).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3 + CC49).)
References
Lottich SC et. al. Breast Cancer Research and Treatment, 1985, 6(1):49-56.Thor A et. al. Cancer Research, 1986, 46(6):3118-24
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Product Notes
The TAG-72 (Catalog #AAA62630) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody reacts with Human, Cow, Dog, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TAG-72 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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