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ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TARS mAb. [Lot No. TARSF8H3-1])
Mouse anti-Human TARS Monoclonal Antibody | anti-TARS antibody
Mouse monoclonal antibody Anti-Human TARS
Gene Names
TARS; ThrRS
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunocytochemistry, Dot Blot
Synonyms
TARS, Antibody; Mouse monoclonal antibody Anti-Human TARS; Homo sapiens threonyl-tRNA synthetase, mRNA (cDNA clone MGC:9344 IMAGE:3458845), complete cds; ThrRS; MGC9344; anti-TARS antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
TARSF8H3
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
2662
Applicable Applications for anti-TARS antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ICC (Immunocytochemistry), DB (Dot Blot)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TARS mAb. [Lot No. TARSF8H3-1])
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TARS mAb. [Lot No. TARSF8H3-1])
FCM/FACS (Flow Cytometry)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. TARSF8H3-1])
FCM/FACS (Flow Cytometry)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. TARSF8H3-1])
WB (Western Blot)
(Detection of human TARS by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. TARSF8H3-1]Predicted molecular weight: 83 kDa)
WB (Western Blot)
(Detection of human TARS by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. TARSF8H3-1]Predicted molecular weight: 83 kDa)
Quality Control
(Left: Western blot analysis of immunized recombinant protein, using anti-TARS monoclonal antibody.)
Quality Control
(Left: Western blot analysis of immunized recombinant protein, using anti-TARS monoclonal antibody.)
Related Product Information for anti-TARS antibody
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family [NCBI Entrez Gene Summary]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
86,861 Da
NCBI Official Full Name
Homo sapiens threonyl-tRNA synthetase, mRNA
NCBI Official Synonym Full Names
threonyl-tRNA synthetase
NCBI Official Symbol
TARS
NCBI Official Synonym Symbols
ThrRS
NCBI Protein Information
threonine--tRNA ligase, cytoplasmic; threonine tRNA ligase 1, cytoplasmic; threonyl-tRNA synthetase, cytoplasmic
UniProt Protein Name
Threonine--tRNA ligase, cytoplasmic
UniProt Gene Name
TARS
UniProt Entry Name
SYTC_HUMAN
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Product Notes
The TARS tars (Catalog #AAA36368) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human TARS reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TARS can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ICC (Immunocytochemistry), DB (Dot Blot). Researchers should empirically determine the suitability of the TARS tars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TARS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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