Mouse TBP-1/PSMC3 Monoclonal Antibody | anti-PSMC3 antibody

Anti-TBP-1/PSMC3 Antibody Picoband (monoclonal, 4D3)

Cat. Num. AAA126954

• Gene Names: PSMC3; TBP1
• Reactivity: Human, Mouse, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: TBP-1/PSMC3, Antibody; Anti-TBP-1/PSMC3 Antibody Picoband (monoclonal, 4D3); anti-PSMC3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 4D3
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-PSMC3 antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: E Coli-derived human TBP-1/PSMC3 recombinant protein (Position: M1-A439).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

FCM/FACS (Flow Cytometry)

(Figure 5. Flow Cytometry analysis of U937 cells using anti-TBP-1/PSMC3 antibody (AAA126954).Overlay histogram showing U937 cells stained with AAA126954 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TBP-1/PSMC3 Antibody (AAA126954, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence)

(Figure 4. IF analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (AAA126954).TBP-1/PSMC3 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-TBP-1/PSMC3 Antibody (AAA126954) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemisry)

(Figure 3. IHC analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (AAA126954).TBP-1/PSMC3 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-TBP-1/PSMC3 Antibody (AAA126954) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohiostchemistry)

(Figure 2. IHC analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (AAA126954).TBP-1/PSMC3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-TBP-1/PSMC3 Antibody (AAA126954) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of TBP-1/PSMC3 using anti-TBP-1/PSMC3 antibody (AAA126954).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human PC-3 whole cell lysates,Lane 5: rat stomach tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse stomach tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TBP-1/PSMC3 antigen affinity purified monoclonal antibody (#AAA126954) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TBP-1/PSMC3 at approximately 50 kDa. The expected band size for TBP-1/PSMC3 is at 50 kDa.)

Related Product Information for anti-PSMC3 antibody

26S protease regulatory subunit 6A, also known as 26S proteasome AAA-ATPase subunit Rpt5, is an enzyme that in humans is encoded by the PSMC3 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.

Product Categories/Family for anti-PSMC3 antibody

Primary Antibodies, Mouse Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 21361144
• NCBI GeneID: 5702
• NCBI Accession #: NP_002795.2
• NCBI GenBank Nucleotide #: NM_002804.4
• UniProt Accession #: P17980; Q3B757; Q3B865; Q53HU5; Q6GPG8; Q6IBS1; Q96HD3; B2R8V1
• Molecular Weight: 49,204 Da
• NCBI Official Full Name: 26S protease regulatory subunit 6A
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, ATPase, 3
• NCBI Official Symbol: PSMC3
• NCBI Official Synonym Symbols: TBP1
• NCBI Protein Information: 26S protease regulatory subunit 6A; TBP-1; Tat-binding protein 1; proteasome subunit P50; proteasome 26S subunit ATPase 3; 26S proteasome AAA-ATPase subunit RPT5; human immunodeficiency virus tat transactivator binding protein-1
• UniProt Protein Name: 26S protease regulatory subunit 6A
• UniProt Gene Name: PSMC3
• UniProt Synonym Gene Names: TBP1; TBP-1
• UniProt Entry Name: PRS6A_HUMAN