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FCM/FACS (Flow Cytometry)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TFAP2A mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 959C2a-1])
Mouse anti-Human TFAP2A Monoclonal Antibody | anti-TFAP2A antibody
Mouse monoclonal antibody Anti-Human TFAP2A
Gene Names
TFAP2A; AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Dot Blot
Synonyms
TFAP2A, Antibody; Mouse monoclonal antibody Anti-Human TFAP2A; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); AP-2; AP2TF; TFAP2; AP-2alpha; anti-TFAP2A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
959C2a
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
3300
Applicable Applications for anti-TFAP2A antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), DB (Dot Blot)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
FCM/FACS (Flow Cytometry)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TFAP2A mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 959C2a-1])
FCM/FACS (Flow Cytometry)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TFAP2A mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 959C2a-1])
WB (Western Blot)
(Detection of TFAP2A by Western blot.Samples: Whole cell lysate from human HEK293 (H, 50 ug) and mouse NIH3T3 (M, 50 ug) cells. [Lot No. 959C2a-1]Predicted molecular weight: 48 kDa)
WB (Western Blot)
(Detection of TFAP2A by Western blot.Samples: Whole cell lysate from human HEK293 (H, 50 ug) and mouse NIH3T3 (M, 50 ug) cells. [Lot No. 959C2a-1]Predicted molecular weight: 48 kDa)
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-TFAP2A monoclonal antibody.)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-TFAP2A monoclonal antibody.)
Related Product Information for anti-TFAP2A antibody
AP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.[supplied by OMIM] [NCBI Entrez Gene Summary]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,440 Da
NCBI Official Full Name
Homo sapiens transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) (TFAP2A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
NCBI Official Symbol
TFAP2A
NCBI Official Synonym Symbols
AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
NCBI Protein Information
transcription factor AP-2-alpha; AP-2 transcription factor; AP2-alpha; activating enhancer-binding protein 2-alpha; activator protein 2
UniProt Protein Name
Transcription factor AP-2-alpha
UniProt Gene Name
TFAP2A
UniProt Entry Name
AP2A_HUMAN
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Product Notes
The TFAP2A tfap2a (Catalog #AAA36359) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human TFAP2A reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TFAP2A can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), DB (Dot Blot). Researchers should empirically determine the suitability of the TFAP2A tfap2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFAP2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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