TGF-beta 1 Recombinant Antibody | TGF-beta1 recombinant antibody

Rabbit anti-TGF-beta 1 Recombinant Monoclonal Antibody [BLR195J]

Cat. Num. AAA23863

• Gene Names: TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: TGF-beta 1; Monoclonal Recombinant Antibody; Rabbit anti-TGF-beta 1 Recombinant Monoclonal Antibody [BLR195J]; LAP; TGF-beta-1; TGFbeta; transforming growth factor beta-1; TGFB; DPD1; CED; prepro-transforming growth factor beta-1; latency-associated peptide; Transforming growth factor beta-1; transforming growth factor beta 1; TGF-beta1 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR195J
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for TGF-beta1 recombinant antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 ul per 1 x 10^6 cells.
• Immunogen: Between 340 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse TGF-beta 1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, Ba/F3, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TGF-beta 1 recombinant monoclonal antibody (AAA23863 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of human TGF-beta 1 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-8, K-562, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TGF-beta 1 recombinant monoclonal antibody (AAA23863 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IHC (Immunohistchemistry)

(Detection of mouse TGF-beta 1 by immunohistochemistry. Sample: FFPE section of bone marrow. Antibody: Rabbit anti-TGF-beta 1 recombinant monoclonal antibody (AAA23863 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of human TGF-beta 1 by immunohistochemistry. Sample: FFPE section of acute myeloid leukemia (AML). Antibody: Rabbit anti-TGF-beta 1 recombinant monoclonal antibody (AAA23863 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of human TGF-beta 1 by immunohistochemistry. Sample: FFPE section of osteosarcoma. Antibody: Rabbit anti-TGF-beta 1 recombinant monoclonal antibody (AAA23863 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of mouse TGF-beta 1 by immunocytochemistry. Sample: FFPE section of Ba/F3. Antibody: Rabbit anti-TGF-beta 1 recombinant monoclonal antibody (AAA23863 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of human TGF-beta 1 by immunocytochemistry. Sample: FFPE section of THP-1 cells treated with PMA (left) and THP-1 cells untreated (right). Antibody: Rabbit anti-TGF-beta 1 recombinant monoclonal antibody (AAA23863 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of human TGF-beta 1 (shaded) in OVCAR8 cells by flow cytometry. Antibody: Rabbit anti-TGF-beta 1 recombinant monoclonal antibody (AAA23863) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for TGF-beta1 recombinant antibody

The Transforming growth factor beta-1 preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature TGF-beta 1 peptide. TGF-beta 1 bound to LAP is inactive, while the active form consists solely of the mature peptide homodimer. The mature TGF-beta 1 peptide may also form heterodimers with other TGFB family members. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. TGF-beta 1 regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells. [taken from NCBI Entrez Gene (Gene ID: 7040)].

Product Categories/Family for TGF-beta1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 63025222
• NCBI GeneID: 7040
• NCBI Accession #: NP_000651.3
• NCBI GenBank Nucleotide #: NM_000660.4
• UniProt Accession #: P01137; Q9UCG4; A8K792
• Molecular Weight: 44,341 Da
• NCBI Official Full Name: transforming growth factor beta-1
• NCBI Official Synonym Full Names: transforming growth factor, beta 1
• NCBI Official Symbol: TGFB1
• NCBI Official Synonym Symbols: CED; LAP; DPD1; TGFB; TGFbeta
• NCBI Protein Information: transforming growth factor beta-1; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide
• UniProt Protein Name: Transforming growth factor beta-1
• UniProt Gene Name: TGFB1
• UniProt Synonym Gene Names: TGFB; TGF-beta-1; LAP
• UniProt Entry Name: TGFB1_HUMAN