Standard Curve (Sample)
General TGF-beta1 (Transforming Growth Factor Beta 1) ELISA Kit | TGF-beta1 elisa kit
TGF-beta1 (Transforming Growth Factor Beta 1) ELISA Kit
Reactivity
General
Synonyms
TGF-beta1 (Transforming Growth Factor Beta 1); N/A; TGF-beta1 (Transforming Growth Factor Beta 1) ELISA Kit; TGF-beta1; TGF-beta1 elisa kit
Reactivity
General
Specificity
This kit recognizes TGF-beta1 in samples. No significant cross-reactivity or interference between TGF-beta1 and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
0.16-10ng/mL
Sensitivity
0.1ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level TGF-beta1 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level TGF-beta1 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TGF-beta1 elisa kit
Background: Transforming Growth Factor Beta-1 (TGF-beta1) is a pleiotropic cytokine with potent anti-inflammatory property, and has been considered an essential risk factor in the inflammatory process of Ischemic Stroke (IS) by involving in the pathophysiological progression of lipid metabolisms, hypertension, and atherosclerosis [1]. TGF-beta1 gene is located on chromosome 19 (q13.1-13.3), including 7 exons and 6 introns. The TGFbeta-1 protein is found throughout the body but is particularly abundant in tissues that make up the skeleton, where it helps regulate the formation and growth of bone and cartilage, a tough, flexible tissue that makes up much of the skeleton during early development. TGFbeta-1 is also involved in the formation of blood vessels, development of muscle tissue and body fat, wound healing, inflammatory processes in the immune system, and prevention of tumor growth [2].
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF-beta1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF-beta1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro-plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-beta1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of TGF-beta1. You can calculate the concentration of TGF-beta1 in the samples by comparing the OD of the samples to the standard curve.
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of TGF-beta1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF-beta1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF-beta1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro-plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-beta1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of TGF-beta1. You can calculate the concentration of TGF-beta1 in the samples by comparing the OD of the samples to the standard curve.
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of TGF-beta1 concentrations in serum, plasma and other biological fluids.
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Product Notes
The General TGF-beta1 (Catalog #AAA177845) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177845 ELISA Kit recognizes General TGF-beta1. It is sometimes possible for the material contained within the vial of "TGF-beta1 (Transforming Growth Factor Beta 1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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