Mouse Thomsen-Friedenreich Antigen/CD176 Monoclonal Antibody

Thomsen-Friedenreich Antigen/CD176 (Pan Carcinoma Marker)

Cat. Num. AAA215343

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Synonyms: Thomsen-Friedenreich Antigen/CD176, Antibody; Thomsen-Friedenreich Antigen/CD176 (Pan Carcinoma Marker); T-F Antigen; TF Antigen; Asialoglycophorin; pan-carcinoma marker; CD176; anti-Thomsen-Friedenreich Antigen/CD176 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: A63-C/A9
• Specificity: Recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-Thomsen-Friedenreich Antigen/CD176 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence)
• Positive Control: KG1 cells. Human colorectal carcinoma tissues.
• Immunogen: Neuraminidase-treated human red blood cells
• Cellular Localization: Cell Surface
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with T-F Antigen Mouse Monoclonal Antibody (A63-C/A9).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with T-F Antigen Mouse Monoclonal Antibody (A63-C/A9).)

References

1. Ryder SD et al. Gastroenterology 1998, 114(1):44-9. 2. Karsten U et al. Hybridoma 1995, 14(1):37-44. 3. Baldus SE et al. Cancer 1998, 82(6):1019-27