WB (Western Blot)
(Detection of mouse Thrombomodulin by western blot. Samples: Whole cell lysate (50 ug) from mIMCD-3, NIH 3T3, bEnd.3, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (AAA213671 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
Thrombomodulin Recombinant Antibody | Thbd recombinant antibody
Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody [BLR260L]
Gene Names
Thbd; TM; CD141; AI385582
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Thrombomodulin, Recombinant Antibody; Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody [BLR260L]; CD141; TM; thrombomodulin; fetomodulin; CD antigen CD141; AI385582; snoRNA MBII-339; Thrombomodulin; Thbd recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Clone Number
BLR260L
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
500ug/ml (varies by lot)
Applicable Applications for Thbd recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 17-517 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse Thrombomodulin by western blot. Samples: Whole cell lysate (50 ug) from mIMCD-3, NIH 3T3, bEnd.3, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (AAA213671 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse Thrombomodulin by western blot. Samples: Whole cell lysate (50 ug) from mIMCD-3, NIH 3T3, bEnd.3, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (AAA213671 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
ICC (Immunocytochemistry)
(Detection of mouse Thrombomodulin by immunocytochemistry. Sample: FFPE section of mouse 7OZ/3 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (AAA213671 lot 1) used for IP at 12 ul/mg lysate. Thrombomodulin was also immunoprecipitated by a second antibody against a different epitope of Thrombomodulin (Antibody 2). For blotting immunoprecipitated Thrombomodulin, AAA213671 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)
ICC (Immunocytochemistry)
(Detection of mouse Thrombomodulin by immunocytochemistry. Sample: FFPE section of mouse 7OZ/3 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (AAA213671 lot 1) used for IP at 12 ul/mg lysate. Thrombomodulin was also immunoprecipitated by a second antibody against a different epitope of Thrombomodulin (Antibody 2). For blotting immunoprecipitated Thrombomodulin, AAA213671 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)
FCM/FACS (Flow Cytometry)
(Detection of mouse Thrombomodulin (shaded) in 7OZ/3 cells by flow cytometry. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (AAA213671) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of mouse Thrombomodulin (shaded) in 7OZ/3 cells by flow cytometry. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (AAA213671) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for Thbd recombinant antibody
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P07204].
Product Categories/Family for Thbd recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,868 Da
NCBI Official Full Name
thrombomodulin
NCBI Official Synonym Full Names
thrombomodulin
NCBI Official Symbol
Thbd
NCBI Official Synonym Symbols
TM; CD141; AI385582
NCBI Protein Information
thrombomodulin
UniProt Protein Name
Thrombomodulin
UniProt Gene Name
Thbd
UniProt Synonym Gene Names
TM
UniProt Entry Name
TRBM_MOUSE
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Product Notes
The Thbd thbd (Catalog #AAA213671) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Thrombomodulin Recombinant Monoclonal Antibody [BLR260L] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Thrombomodulin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Thbd thbd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thrombomodulin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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