TIGIT recombinant antibody

Rabbit anti-TIGIT Recombinant Monoclonal Antibody [BLR047F]

Cat. Num. AAA213546

• Gene Names: TIGIT; VSIG9; VSTM3; WUCAM
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: TIGIT, Recombinant Antibody; Rabbit anti-TIGIT Recombinant Monoclonal Antibody [BLR047F]; VSTM3; Washington University cell adhesion molecule; VSIG9, VSTM3; VSIG9; V-set and transmembrane domain containing 3; V-set and immunoglobulin domain-containing protein 9; V-set and immunoglobulin domain containing 9; T-cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain-containing protein 3; WUCAM; T cell immunoreceptor with Ig and ITIM domains; TIGIT recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: BLR047F
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for TIGIT recombinant antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Between 194 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human TIGIT by western blot. Samples: Whole cell lysate (10 ug) from HeLa, My-La CD4+, HEK293T, MJ, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (AAA213546 lot 6) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IHC (Immunohistochemisry)

(Detection of human TIGIT (red) by immunohistochemistry. Sample:FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (AAA213546 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohiostchemistry)

(Detection of human TIGIT by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (AAA213546 lot 1). Secondary: Dylight 594 conjugated goat-anti-rabbit IgG . Counterstain: DAPI (blue).)

FCM/FACS (Flow Cytometry)

(Detection of human TIGIT (shaded) in MJ cells by flow cytometry. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (AAA213546) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Related Product Information for TIGIT recombinant antibody

TIGIT is a member of the PVR (poliovirus receptor) family of immunoglobin proteins and is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. [taken from NCBI Entrez Gene (Gene ID: 201633)].

Product Categories/Family for TIGIT recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 256600228
• NCBI GeneID: 201633
• NCBI Accession #: NP_776160.2
• NCBI GenBank Nucleotide #: NM_173799.3
• UniProt Accession #: Q495A1; Q495A3; Q5JPD8; Q6MZS2; Q8N877
• Molecular Weight: 18,432 Da
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: TIGIT
• NCBI Official Synonym Symbols: VSIG9; VSTM3; WUCAM
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain containing 3; V-set and immunoglobulin domain containing 9; Washington University cell adhesion molecule; V-set and transmembrane domain-containing protein 3; V-set and immunog
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: TIGIT
• UniProt Synonym Gene Names: VSIG9; VSTM3