<
WB (Western Blot)
(Detection of human TIM3 by western blot. Samples: Whole cell lysate (10 ug) from RPMI-8226, HeLa, SR, HEK293T, and SUP-M2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody [BL-347-6E6] (AAA23803 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
TIM3 recombinant antibody
Rabbit anti-TIM3 Recombinant Monoclonal Antibody [BLR033F]
Gene Names
HAVCR2; TIM3; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
TIM3, Recombinant Antibody; Rabbit anti-TIM3 Recombinant Monoclonal Antibody [BLR033F]; T-cell membrane protein 3; TIMD-3; TIMD3; TIM3; T-cell immunoglobulin mucin receptor 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin and mucin domain-containing protein 3; KIM-3; kidney injury molecule-3; hepatitis A virus cellular receptor 2; HAVcr-2; CD366; CD antigen CD366; T cell immunoglobulin mucin 3; Tim-3; Hepatitis A virus cellular receptor 2; TIM3 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR033F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for TIM3 recombinant antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Immunogen
Residues 22-201 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human TIM3 by western blot. Samples: Whole cell lysate (10 ug) from RPMI-8226, HeLa, SR, HEK293T, and SUP-M2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody [BL-347-6E6] (AAA23803 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human TIM3 by western blot. Samples: Whole cell lysate (10 ug) from RPMI-8226, HeLa, SR, HEK293T, and SUP-M2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody [BL-347-6E6] (AAA23803 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IHC (Immunohistochemistry)
(Detection of human TIM3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody (AAA23803) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human TIM3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody (AAA23803) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human TIM3 by immunohistochemistry. Sample: FFPE section of pleural effusion lung carcinoma. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody (AAA23803 lot 2) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human TIM3 by immunohistochemistry. Sample: FFPE section of pleural effusion lung carcinoma. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody (AAA23803 lot 2) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human TIM3 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody (AAA23803 lot 2) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human TIM3 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody (AAA23803 lot 2) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human TIM3 by immunocytochemistry. Sample: FFPE section of RPMI-8226 cells. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody (AAA23803 lot 2) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human TIM3 by immunocytochemistry. Sample: FFPE section of RPMI-8226 cells. Antibody: Rabbit anti-TIM3 recombinant monoclonal antibody (AAA23803 lot 2) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human TIM3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-TIM3 recombinant monoclonal (AAA23803) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human TIM3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-TIM3 recombinant monoclonal (AAA23803) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for TIM3 recombinant antibody
TIM3 (HAVCR2) is a cell surface receptor implicated in modulating innate and adaptive immune responses. It is generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. TIM3 regulates macrophage activation, inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses, and promotes immunological tolerance. In CD8+ cells TIM3 attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8TDQ0].
Product Categories/Family for TIM3 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,149 Da
NCBI Official Full Name
hepatitis A virus cellular receptor 2
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 2
NCBI Official Symbol
HAVCR2
NCBI Official Synonym Symbols
TIM3; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
NCBI Protein Information
hepatitis A virus cellular receptor 2; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell m
UniProt Protein Name
Hepatitis A virus cellular receptor 2
UniProt Gene Name
HAVCR2
UniProt Synonym Gene Names
TIM3; TIMD3; HAVcr-2; TIMD-3; TIM-3
UniProt Entry Name
HAVR2_HUMAN
Similar Products
Product Notes
The TIM3 havcr2 (Catalog #AAA23803) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TIM3 Recombinant Monoclonal Antibody [BLR033F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIM3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the TIM3 havcr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIM3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.