IF (Immunofluorescence)
(IF analysis of TJP1 using anti-TJP1 antibody (AAA125386).TJP1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (please inquire) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2ug/mL mouse anti-TJP1 Antibody (AAA125386) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Mouse anti-Human TJP1 Monoclonal Antibody | anti-TJP1 antibody
Anti-TJP1 Antibody (Monoclonal, 3E12)
Gene Names
TJP1; ZO-1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
TJP1, Antibody; Anti-TJP1 Antibody (Monoclonal, 3E12); Tight junction protein ZO-1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; TJP1; ZO1; anti-TJP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3E12
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
1748
Applicable Applications for anti-TJP1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E. coli-derived human TJP1 recombinant protein (Position: H1178-F1527). Human TJP1 shares 82% amino acid (aa) sequence identity with mouse TJP1.
Relevant Detection Systems
AAA Biotech recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (please inquire) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (please inquire) for IHC(P).
Preparation and Storage
Store at -20°C for one year. After reconstitution, store at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(IF analysis of TJP1 using anti-TJP1 antibody (AAA125386).TJP1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (please inquire) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2ug/mL mouse anti-TJP1 Antibody (AAA125386) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(IF analysis of TJP1 using anti-TJP1 antibody (AAA125386).TJP1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (please inquire) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2ug/mL mouse anti-TJP1 Antibody (AAA125386) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
FCM/FACS (Flow Cytometry)
(TJP1 antibody (AAA125386).Overlay histogram showing hela cells stained with AAA125386 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TJP1 Antibody (AAA125386, 1ug/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220 kDa. The expected band size for TJP1 is at 195 kDa.)
FCM/FACS (Flow Cytometry)
(TJP1 antibody (AAA125386).Overlay histogram showing hela cells stained with AAA125386 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TJP1 Antibody (AAA125386, 1ug/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220 kDa. The expected band size for TJP1 is at 195 kDa.)
Related Product Information for anti-TJP1 antibody
Description: Anti-TJP1 Antibody Picoband™ (monoclonal, 3E12) catalog # MBS1752895. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
References
1. Mohandas TK, Chen XN, Rowe LB, Birkenmeier EH, Fanning AS, Anderson JM, Korenberg JR (February 1997). "Localization of the tight junction protein gene TJP1 to human chromosome 15q13, distal to the Prader-Willi/Angelman region, and to mouse chromosome 7". Genomics 30 (3): 594-7. 2. Willott, E., Balda, M. S., Fanning, A. S., Jameson, B., Van Itallie, C., Anderson, J. M. The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions. Proc. Nat. Acad. Sci. 90: 7834-7838, 1993.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Tight junction protein ZO-1
NCBI Official Synonym Full Names
tight junction protein 1
NCBI Official Symbol
TJP1
NCBI Official Synonym Symbols
ZO-1
NCBI Protein Information
tight junction protein ZO-1
UniProt Protein Name
Tight junction protein ZO-1
UniProt Gene Name
TJP1
UniProt Synonym Gene Names
ZO1
UniProt Entry Name
ZO1_HUMAN
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Product Notes
The TJP1 tjp1 (Catalog #AAA125386) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-TJP1 Antibody (Monoclonal, 3E12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TJP1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TJP1 tjp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TJP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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