WB (Western Blot)
(Detection of human TJP1/ZO-1 by western blot. Samples: Whole cell lysate (25 ug) from A-549, HeLa, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal antibody (AAA23830A lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
TJP1/ZO-1 recombinant antibody
Rabbit anti-TJP1/ZO-1 Recombinant Monoclonal Antibody [BLR092G]
IP: 12 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human TJP1/ZO-1 by western blot. Samples: Whole cell lysate (25 ug) from A-549, HeLa, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal antibody (AAA23830A lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human TJP1/ZO-1 by western blot. Samples: Whole cell lysate (25 ug) from A-549, HeLa, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal antibody (AAA23830A lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse TJP1/ZO-1 by western blot. Samples: Whole cell lysate (25 ug) from mIMCD-3, RenCa, TCMK-1, NIH 3T3, and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal antibody (AAA23830A lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse TJP1/ZO-1 by western blot. Samples: Whole cell lysate (25 ug) from mIMCD-3, RenCa, TCMK-1, NIH 3T3, and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal antibody (AAA23830A lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human TJP1/ZO-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-TJP1/ZO-1 recombinant monoclonal antibody (AAA23830A lot 3) used for IP at 20 ul/mg lysate. TJP1/ZO-1 was also immunoprecipitated by a previous lot of this antibody (AAA23830A lot 2) and a second antibody against a different epitope of TJP1/ZO-1 (BL18749). For blotting immunoprecipitated TJP1/ZO-1, AAA23830A was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human TJP1/ZO-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-TJP1/ZO-1 recombinant monoclonal antibody (AAA23830A lot 3) used for IP at 20 ul/mg lysate. TJP1/ZO-1 was also immunoprecipitated by a previous lot of this antibody (AAA23830A lot 2) and a second antibody against a different epitope of TJP1/ZO-1 (BL18749). For blotting immunoprecipitated TJP1/ZO-1, AAA23830A was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human TJP1/ZO-1 in FFPE kidney by immunohistochemistry-IF. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal antibody (AAA23830). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry)
(Detection of human TJP1/ZO-1 in FFPE kidney by immunohistochemistry-IF. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal antibody (AAA23830). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry)
(Detection of human ZO-1 in FFPE kidney by IHC. Antibody: Rabbit anti-ZO-1 recombinant monoclonal antibody (AAA23830 Lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human ZO-1 in FFPE kidney by IHC. Antibody: Rabbit anti-ZO-1 recombinant monoclonal antibody (AAA23830 Lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human ZO-1 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-ZO-1 recombinant monoclonal antibody (AAA23830 Lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human ZO-1 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-ZO-1 recombinant monoclonal antibody (AAA23830 Lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human ZO-1 in FFPE A549 cells by ICC. Antibody: Rabbit anti-ZO-1 recombinant monoclonal antibody (AAA23830 Lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human ZO-1 in FFPE A549 cells by ICC. Antibody: Rabbit anti-ZO-1 recombinant monoclonal antibody (AAA23830 Lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
NCBI and Uniprot Product Information
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Product Notes
The TJP1/ZO-1 tjp1 (Catalog #AAA23830) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TJP1/ZO-1 Recombinant Monoclonal Antibody [BLR092G] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TJP1/ZO-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF). WB: 1:1000 IP: 12 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Researchers should empirically determine the suitability of the TJP1/ZO-1 tjp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TJP1/ZO-1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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