Goat ZO-1 Polyclonal Antibody | anti-ZO-1 antibody

ZO-1 Polyclonal Antibody

Cat. Num. AAA13861

• Gene Names: Tjp1; ZO1
• Reactivity: Reacts against Human, Rat, Mouse, Canine and Monkey proteins.
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Epitope-affinity purified from goat antiserum
• Synonyms: ZO-1, Antibody; ZO-1 Polyclonal Antibody; ZO-1 and zona occludens protein 1 antibody; anti-ZO-1 antibody

• Host: Goat
• Reactivity: Reacts against Human, Rat, Mouse, Canine and Monkey proteins.
• Clonality: Polyclonal
• Specificity: Detects endogenous levels of total ZO-1 by Western blot in whole cell and tissue lysates.
• Purity/Purification: Epitope-affinity purified from goat antiserum
• Form/Format: Polyclonal antibody supplied as a 200 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.

• Applicable Applications for anti-ZO-1 antibody: WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Application Notes: Western blot: 1:500-1:2,000; lmmunofluorescence: 1:50-1:250; Immunohistochemistry (paraffin): 1:200-1:1000; Immunohistochemistry (frozen): 1:200-1:1000; ; Special instructions: The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles..
• Immunogen: Purified recombinant peptide derived from within residues 1580 aa to the C-terminus of human ZO-1 produced in E. coli.
• Preparation and Storage: For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.

IHC (Immunohistochemistry)

(IHC of Mouse kidney using Z01 antibody and FFPE tissue after heat-induced retrieval. Anti-Z01 Ab at 1:750/DAB detection.)

IHC (Immunohistochemistry)

(IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.)

IHC (Immunohistchemistry)

(IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.)

IF (Immunofluorescence)

(Immunofluorescence of primary RPE cells with ZO1 Ab at 1/100 dilution.)

IF (Immunofluorescence)

(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)

IF (Immunofluorescence)

(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)

IF (Immunofluorescence)

(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)

IF (Immunofluorescence)

(Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.)

WB (Western Blot)

(Anti-ZO1 Ab at 1/2,500 dilution; 50ug of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.)

Immunoperoxidase

(Immunoperoxidase of polyclonal antibody to ZO-1 (1/200) on paraformaldehyde-fixed paraffin-embedded mouse kidney.)

Reactivity Chart

Related Product Information for anti-ZO-1 antibody

Goat polyclonal to TJPl tight Junction protein 1 (TJP1). TJPl is located on a cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions.

NCBI and Uniprot Product Information

• NCBI GI #: 303710
• NCBI GeneID: 21872
• UniProt Accession #: P39447; E9QK00
• NCBI Official Full Name: ZO-1
• NCBI Official Synonym Full Names: tight junction protein 1
• NCBI Official Symbol: Tjp1
• NCBI Official Synonym Symbols: ZO1
• NCBI Protein Information: tight junction protein ZO-1; zona occludens protein 1; zonula occludens protein 1
• UniProt Protein Name: Tight junction protein ZO-1
• UniProt Gene Name: Tjp1
• UniProt Synonym Gene Names: Zo1
• UniProt Entry Name: ZO1_MOUSE

Frequently Asked Questions

What is ZO-1 used in tight junction studies?

ZO-1 is used as a marker protein to study tight junctions between cells. Researchers use it to see how cells stick together and form barriers, especially in epithelial and endothelial tissues. It reveals if tight junctions are normal or damaged.

Can a ZO-1 antibody be used to assess epithelial or endothelial barrier integrity?

Yes, a ZO-1 antibody is commonly used to check barrier strength in epithelial and endothelial cells. Weak or broken ZO-1 staining means a damaged, leaky barrier. Strong, continuous staining shows a healthy barrier.

Is ZO-1 expression altered during inflammation or cancer progression?

Yes, ZO-1 expression often changes during inflammation and cancer. It may decrease, move away from cell junctions, or become irregular. These changes indicate loss of tight junctions, which is common when tissues become inflamed or cancer cells start spreading.

Can ZO-1 antibody be used for immunofluorescence to visualize cell junctions?

Yes, the ZO-1 antibody can be used for immunofluorescence. It allows researchers to visually see tight junctions under a microscope by glowing at cell borders, making it easy to study junction structure and location.

How does ZO-1 staining change during epithelial–mesenchymal transition (EMT)?

During EMT, ZO-1 staining weakens or becomes discontinuous. The signal spreads or vanishes instead of clear cell-border lines, showing tight junction loss as cells turn mobile and invasive.

Is ZO-1 commonly studied in gut, lung, or blood–brain barrier research?

Yes, ZO-1 is widely studied in the gut, lungs, and blood–brain barrier. These tissues rely on strong tight junctions. Researchers use ZO-1 antibodies to study barrier function, permeability changes, and disease-related damage in these organs.