Mouse TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody | anti-TNF antibody

TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody

Cat. Num. AAA13806

• Gene Names: TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
• Reactivity: Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish
• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
• Synonyms: TNF-alpha (Tumor Necrosis Factor alpha), Antibody; TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody; APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor; anti-TNF antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: TNF706
• Specificity: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein L. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
• Sequence Length: 233

• Applicable Applications for anti-TNF antibody: Flow Cytometry (FC), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin
• Application Notes: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-paraffin) (2-4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Cellular Localization: Cytoplasmic and extracellular (Secreted)
• Immunogen: Recombinant N-terminal fragment of human TNF-alpha
• Hu-Chromosome Location: 6p21.33
• Positive Control: HePG2, HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil (IHC).
• Preparation and Storage: Antibody with azide - store at 2 to 8 °C. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescence staining of PFA-fixed HePG2 cells using Tumor Necrosis Factor (TNF alpha) Mouse Monoclonal Antibody (TNF706) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded rat pancreas stained with TNF alpha Mouse Monoclonal Antibody (TNF706).)

IHC (Immunohistochemistry)

(Formalin paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNF706).)

References

McLaughlin PJ; Elwood NJ; Ramadi LT, Pica MR, McKenzie IF. Improvement in sensitivity of enzyme-linked immunosorbent assay for tumor necrosis factor. Immunol Cell Biol, 1990, 68:51-5.

NCBI and Uniprot Product Information

• NCBI GI #: 25952111
• NCBI GeneID: 7124
• NCBI Accession #: NP_000585.2
• NCBI GenBank Nucleotide #: NM_000594.3
• UniProt Accession #: P01375; O43647; Q9P1Q2; Q9UIV3
• Molecular Weight: 17kDa
• NCBI Official Full Name: tumor necrosis factor
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
• NCBI Protein Information: tumor necrosis factor
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_HUMAN