TNF alpha elisa kit

Murine TNF alpha ELISA Kit

Cat. Num. AAA58598

• Gene Names: TNF; DIF; TNFA; TNFSF2; TNF-alpha
• Reactivity: Murine. No cross reactivity with other murine cytokines.
• Synonyms: TNF alpha; N/A; Murine TNF alpha ELISA Kit; TNF-a; TNF alpha elisa kit

• Reactivity: Murine. No cross reactivity with other murine cytokines.
• Specificity: The assay recognizes both natural and recombinant murine TNFalpha and rat TNFalpha. To define the specificity of this ELISA several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested: human TNFalpha, murine IL-2, murine IL-6, murine IL-10, murine IL-23, murine IFNgamma, murine GM-CSF, murine CD138.
• Sequence Length: 233
• Assay Type: Qualitative Sandwich
• Samples: Supernatants, buffered solutions or serum and plasma samples
• Sensitivity: 10.7 pg/ml

• Intra-assay Precision: Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) of samples containing different concentrations of TNFalpha: 2 in murine pooled Serum, 2 in Culture Media and 2 in Standard Diluent. Data below show the mean mTNFalpha concentration and the coefficient of variation for each sample.
• Inter-assay Precision: Assay to assay reproducibility within one laboratory was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) of samples containing different concentrations of mTNFalpha: 2 in murine pooled Serum, 2 in Culture Media and 2 in Standard Diluent. Data below show the mean mTNFalpha concentration and the coefficient of variation for each sample.

Standard Curve (Sample)

Related Product Information for TNF alpha elisa kit

Principle of the Assay: A capture Antibody highly specific for mTNFalpha has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of mTNFalpha samples and known standards to the capture antibodies and subsequent binding of the Biotinylated anti-mTNFalpha secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of mTNFalpha present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of mTNFalpha in any sample tested.

Intended Uses: The Murine TNFalpha ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of murine TNFalpha (mTNFalpha) in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant mTNFalpha.

Product Categories/Family for TNF alpha elisa kit

Mouse ELISA Kit

References

Campolo, M. et al., J. Leukoc. Biol.,2013;93:277-287
Di Paola, R. et al.,J. Leukoc. Biol,2010; 88: 291-301
Esposito, E. et al., J Leukoc Biol.,2007; 81: 1032 - 1043

NCBI and Uniprot Product Information

• NCBI GI #: 37210
• NCBI GeneID: 7124
• UniProt Accession #: P01375; O43647; Q9P1Q2; Q9UIV3
• Molecular Weight: 25,644 Da
• NCBI Official Full Name: TNF-alpha
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: DIF; TNFA; TNFSF2; TNF-alpha
• NCBI Protein Information: tumor necrosis factor
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_HUMAN