Application Data
(TNFR1/TNFRSF1A-mediated inhibition of cytotoxicity was Neutralized by Human TNFR1 Antibody. Recombinant Human TNFR1/TNFRSF1A inhibits Recombinant Human TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. Inhibition of Recombinant Human TNFa (0.2 ng/mL) activity elicited by Recombinant Human TNFR1/TNFRSF1A (0.3 ug/mL) is neutralized by increasing concentrations of Human TNFR1/TNFRSF1A Monoclonal Antibody. The IC50 is typically 0.5-1.5 ug/mL in the presence of the metabolic inhibitor actinomycin D (1 ug/mL).)
Rabbit TNFR1/CD120a/TNFRSF1A Monoclonal Antibody | anti-TNFRSF1A antibody
TNFR1/CD120a/TNFRSF1A Neutralizing Antibody
Gene Names
TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
Applications
Neutralization Assay
Synonyms
TNFR1/CD120a/TNFRSF1A, Antibody; TNFR1/CD120a/TNFRSF1A Neutralizing Antibody; tumor necrosis factor receptor superfamily member 1A; Anti-CD120a Antibody; Anti-FPF Antibody; Anti-MS5 Antibody; Anti-p55 Antibody; Anti-p55-R Antibody; Anti-p60 Antibody; Anti-TBP1 Antibody; Anti-TNF-R Antibody; Anti-TNF-R-I Antibody; Anti-TNF-R55 Antibody; Anti-TNFAR Antibody; Anti-TNFR1 Antibody; Anti-TNFR1-d2 Antibody; Anti-TNFR55 Antibody; Anti-TNFR60 Antibody; anti-TNFRSF1A antibody
Host
Rabbit
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R111
Specificity
Human TNFR1/CD120a/TNFRSF1A.
Form/Format
Liquid; 0.2um filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Applicable Applications for anti-TNFRSF1A antibody
Neutralization
Application Notes
Neutralization: The neutralization activity of TNFR1/TNFRSF1A Neutralizing Antibody is Measured by its ability to neutralize TNFR1/TNFRSF1A-mediated inhibition of cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 0.5-1.5ug/mL in the presence of 0.3ug/mL Recombinant Human TNFR1/TNFRSF1A, 0.2ng/mL Recombinant Human TNFa and 1ug/mL actinomycin D.
Immunogen
Recombinant Human TNFR1/CD120a/TNFRSF1A Protein
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human TNFR1/CD120a/TNFRSF1A (rh TNFR1/CD120a/TNFRSF1A ; and was produced using recombinant antibody technology.
Preparation and Storage
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Application Data
(TNFR1/TNFRSF1A-mediated inhibition of cytotoxicity was Neutralized by Human TNFR1 Antibody. Recombinant Human TNFR1/TNFRSF1A inhibits Recombinant Human TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. Inhibition of Recombinant Human TNFa (0.2 ng/mL) activity elicited by Recombinant Human TNFR1/TNFRSF1A (0.3 ug/mL) is neutralized by increasing concentrations of Human TNFR1/TNFRSF1A Monoclonal Antibody. The IC50 is typically 0.5-1.5 ug/mL in the presence of the metabolic inhibitor actinomycin D (1 ug/mL).)
Application Data
(TNFR1/TNFRSF1A-mediated inhibition of cytotoxicity was Neutralized by Human TNFR1 Antibody. Recombinant Human TNFR1/TNFRSF1A inhibits Recombinant Human TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. Inhibition of Recombinant Human TNFa (0.2 ng/mL) activity elicited by Recombinant Human TNFR1/TNFRSF1A (0.3 ug/mL) is neutralized by increasing concentrations of Human TNFR1/TNFRSF1A Monoclonal Antibody. The IC50 is typically 0.5-1.5 ug/mL in the presence of the metabolic inhibitor actinomycin D (1 ug/mL).)
Related Product Information for anti-TNFRSF1A antibody
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1/TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-kappaB, mediate apoptosis, and regulate inflammation processes.
Product Categories/Family for anti-TNFRSF1A antibody
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Cottin V, et al. (2002) Restricted localization of the TNF receptor CD120a to lipid rafts: a novel role for the death domain. The journal of immunology. 168: 4095-102.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 1A
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 1A
NCBI Official Symbol
TNFRSF1A
NCBI Official Synonym Symbols
FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
NCBI Protein Information
tumor necrosis factor receptor superfamily member 1A; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; tumor necrosis factor receptor 1A isoform beta
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 1A
UniProt Gene Name
TNFRSF1A
UniProt Synonym Gene Names
TNFAR; TNFR1; TNF-R1; TNF-RI; TNFR-I; TBPI
UniProt Entry Name
TNR1A_HUMAN
Similar Products
Product Notes
The TNFRSF1A tnfrsf1a (Catalog #AAA27740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNFR1/CD120a/TNFRSF1A can be used in a range of immunoassay formats including, but not limited to, Neutralization. Neutralization: The neutralization activity of TNFR1/TNFRSF1A Neutralizing Antibody is Measured by its ability to neutralize TNFR1/TNFRSF1A-mediated inhibition of cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 0.5-1.5ug/mL in the presence of 0.3ug/mL Recombinant Human TNFR1/TNFRSF1A, 0.2ng/mL Recombinant Human TNFa and 1ug/mL actinomycin D. Researchers should empirically determine the suitability of the TNFRSF1A tnfrsf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFR1/CD120a/TNFRSF1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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