Mouse anti-Human TNPO3 Monoclonal Antibody | anti-TNPO3 antibody

Mouse monoclonal antibody Anti-Human TNPO3

Cat. Num. AAA10612

• Gene Names: TNPO3; IPO12; TRNSR; LGMD1F; MTR10A; TRN-SR; TRN-SR2
• Reactivity: Human
• Applications: Immunocytochemistry, Western Blot
• Synonyms: TNPO3, Antibody; Mouse monoclonal antibody Anti-Human TNPO3; Homo sapiens transportin 3 (TNPO3); IPO12; TRNSR; MTR10A;TRN-SR; TRN-SR2; anti-TNPO3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3152C2a
• Form/Format: Mouse monoclonal anti-human TNPO3 antibody in PBS (3.0 mM KCl, 1.5 mM KH2PO4, 140 mM NaCl, 8.0 mM Na2HPO4(pH7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
• Concentration: 100 ug/mL (varies by lot)
• Sequence Length: 4422

• Applicable Applications for anti-TNPO3 antibody: Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: WB: 0.2-2ug/ml; ICC: 2-100ug/ml
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.
• Disposal: This antibody solution contains sodium azide (NaN3) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
• Immunogen: Recombinant
• Note: Caution must be taken to avoid contact with skin or eyes. In such a case, rinse throughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention inmediately. Wear appropiate protective clothing such as laboratory overalls,safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained laboratory techniques that is is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially, harmful. The vials is prone to fall over. Use caution, especially when the lid is off.
• Preparation and Storage: Store at 2-8°C for up to one year. We recommend storing at –20°C for long-term storage. Avoid repeat freezing and thawing cycles.

ICC (Immunocytochemistry)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TNPO3 mAb.)

WB (Western Blot)

(Detection of TNPO3 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 3152C2a-1]Predicted molecular weight: 104 kDa)

Quality Control

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-TNPO3 monoclonal antibody.)

Related Product Information for anti-TNPO3 antibody

TNPO3 is a nuclear import receptor for serine/arginine-rich (SR) proteins, which are essential precursor-mRNA splicing factors (Kataoka et al., 1999).[supplied by OMIM] [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 300794233
• NCBI GeneID: 23534
• NCBI Accession #: NP_036602.1
• NCBI GenBank Nucleotide #: NM_012470
• NCBI Official Full Name: Homo sapiens transportin 3 (TNPO3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: transportin 3
• NCBI Official Symbol: TNPO3
• NCBI Official Synonym Symbols: IPO12; TRNSR; LGMD1F; MTR10A; TRN-SR; TRN-SR2
• NCBI Protein Information: transportin-3; imp12; importin 12; limb girdle muscular dystrophy 1F (autosomal dominant); transportin-SR
• UniProt Protein Name: Transportin-3
• UniProt Gene Name: TNPO3
• UniProt Entry Name: TNPO3_HUMAN