IF (Immunofluorescence)
(ICC/IF analysis of TP53I3 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TP53I3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human TP53I3 Monoclonal Antibody | anti-TP53I3 antibody
TP53I3 antibody
Gene Names
TP53I3; PIG3
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
TP53I3, Antibody; TP53I3 antibody; Quinone oxidoreductase PIG3; PIG3; Quinone oxidoreductase PIG3 p53 induced gene 3 protein; Putative quinone oxidoreductase; quinone oxidoreductase homolog; TP53I3; Tumor protein p53 inducible protein 3.; anti-TP53I3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT1C9
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
332
Applicable Applications for anti-TP53I3 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human TP53I3 (1-332aa) purified from E. coli
Clone Note
Anti-human TP53I3 mAb, clone AT1C9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TP53I3 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of TP53I3 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TP53I3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of TP53I3 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TP53I3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : SW480 cell lysate The Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: TP53I3 Transfected 293T cell lysate)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : A549 cell lysate Lane 2. : SW480 cell lysate The Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TP53I3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: TP53I3 Transfected 293T cell lysate)
Related Product Information for anti-TP53I3 antibody
TP53I3 participates in the generation of reactive oxygen species (ROS). TP53I3 has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. TP53I3 has low NADPH-dependent diamine reductase activity (in vitro). TP53I3 is localized to the cytoplasm and induced in primary, non-transformed and transformed cell cultures after exposure to genotoxic agents. TP53I3 microsatellite polymorphism is associated with differential susceptibility to cancer.
Product Categories/Family for anti-TP53I3 antibody
References
Porte S, et al. (2009) J Biol Chem.. 284 (25):17194-205 ; Flatt PM. et al. (2000) Cancer Lett. 156 (1):63-72.; J Biol Chem. 2009 Jun 19;284 (25):17194-205.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,402 Da
NCBI Official Full Name
quinone oxidoreductase PIG3 isoform 1
NCBI Official Synonym Full Names
tumor protein p53 inducible protein 3
NCBI Official Symbol
TP53I3
NCBI Official Synonym Symbols
PIG3
NCBI Protein Information
quinone oxidoreductase PIG3
UniProt Protein Name
Quinone oxidoreductase PIG3
UniProt Gene Name
TP53I3
UniProt Synonym Gene Names
PIG3
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Product Notes
The TP53I3 tp53i3 (Catalog #AAA48107) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TP53I3 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TP53I3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TP53I3 tp53i3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TP53I3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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