FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing K562 cells stained with AAA279130 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
TPSB2 recombinant antibody
TPSB2 Recombinant Monoclonal Antibody
Gene Names
TPSAB1; TPS1; TPS2; TPSB1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Affinity purified
Synonyms
TPSB2, Recombinant Antibody; TPSB2 Recombinant Monoclonal Antibody; Tryptase beta-2 (Tryptase-2)(EC 3.4.21.59)(Tryptase II); TPSB2; TPS2; TPSB2 recombinant antibody
Host
HEK293F Cell
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
9H6
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for TPSB2 recombinant antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human TPSB2
Conjugation
Non-conjugated
Research Area
Others
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing K562 cells stained with AAA279130 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing K562 cells stained with AAA279130 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WB (Western Blot)
(Western BlotPositive WB detected in: Mouse Kidney tissue lysate,All lanes: Mast Cell Tryptase antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 30 kDaObserved band size: 30 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Mouse Kidney tissue lysate,All lanes: Mast Cell Tryptase antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 30 kDaObserved band size: 30 kDa)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30kDa
NCBI Official Full Name
tryptase alpha/beta-1
NCBI Official Synonym Full Names
tryptase alpha/beta 1
NCBI Official Symbol
TPSAB1
NCBI Official Synonym Symbols
TPS1; TPS2; TPSB1
NCBI Protein Information
tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase
UniProt Protein Name
Tryptase beta-2
UniProt Gene Name
TPSB2
UniProt Synonym Gene Names
TPS2; Tryptase-2
UniProt Entry Name
TRYB2_HUMAN
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Product Notes
The TPSB2 tpsb2 (Catalog #AAA279130) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The TPSB2 Recombinant Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TPSB2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TPSB2 tpsb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPSB2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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