Application Data
(Figure 3: Neutralizing effect of MAb to TRAIL (human) (2E5) on TRAIL-induced apoptosis (TRAIL, Soluble (human) (recombinant) Set; containing Enhancer for Ligands).")
Mouse TRAIL Monoclonal Antibody | anti-TRAIL antibody
TRAIL (human) monoclonal antibody (2E5)
Does not cross-react with mouse TRAIL
Does not cross-react with mouse TRAIL
Application Data
(Figure 3: Neutralizing effect of MAb to TRAIL (human) (2E5) on TRAIL-induced apoptosis (TRAIL, Soluble (human) (recombinant) Set; containing Enhancer for Ligands).")
Application Data
(Figure 3: Neutralizing effect of MAb to TRAIL (human) (2E5) on TRAIL-induced apoptosis (TRAIL, Soluble (human) (recombinant) Set; containing Enhancer for Ligands).")
Application Data
(Figure 2: MAb to TRAIL (human) (2E5) can be used to detect surface human TRAIL in transiently transfected 293T cells in flow cytometry as well on untransfected cells expressing endogenous surface TRAIL.Method:293T cells (10cm dishes) were transfeced with 0, 1, or 15ug plasmid coding for full length human TRAIL for 24 hours using a standard calcium phosphate transfection method. All dishes were cotransfected with 1ug plasmid coding for EGFPas a cotransfection marker. Viable cells (FSC/SSC region R1), that were GFP-positive (transfected) (FL1 region R3) were analyzed with respect to surface expression of human TRAIL. Cells were stained with MAb to TRAIL (human) (2E5) as well as with an isotype-matched control (control IgG1) at 10ug/ml. Binding of the primary MAbs is detected using RPE conjugated goat anti-mouse IgG (FL2).")
Application Data
(Figure 2: MAb to TRAIL (human) (2E5) can be used to detect surface human TRAIL in transiently transfected 293T cells in flow cytometry as well on untransfected cells expressing endogenous surface TRAIL.Method:293T cells (10cm dishes) were transfeced with 0, 1, or 15ug plasmid coding for full length human TRAIL for 24 hours using a standard calcium phosphate transfection method. All dishes were cotransfected with 1ug plasmid coding for EGFPas a cotransfection marker. Viable cells (FSC/SSC region R1), that were GFP-positive (transfected) (FL1 region R3) were analyzed with respect to surface expression of human TRAIL. Cells were stained with MAb to TRAIL (human) (2E5) as well as with an isotype-matched control (control IgG1) at 10ug/ml. Binding of the primary MAbs is detected using RPE conjugated goat anti-mouse IgG (FL2).")
Application Data
(Figure 1:Apoptosis induced in Jurkat T leukemia cells by Killer TRAILTM is completely blocked by mouse MAb to TRAIL (human) (2E5) in a concentration range of 0.5-1.5ug/ml. Concentration of MAb 2E5 required for inhibition may vary depending of the cell type studied and the concentration of soluble recombinant human TRAIL used. The neutralizing activity of MAb 2E5 has been confirmed with various sources of soluble recombinant human TRAIL including KillerTRAILTM. Method: Jurkat cells (5x104) were treated with KillerTRAILTM (300ng/ml) in the presence or absence of the MAb to TRAIL (human) (2E5) or a control IgG1 mouse monoclonal antibody. After 16 hours apoptosis was evaluated by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analyzed by flow cytometry.")
Application Data
(Figure 1:Apoptosis induced in Jurkat T leukemia cells by Killer TRAILTM is completely blocked by mouse MAb to TRAIL (human) (2E5) in a concentration range of 0.5-1.5ug/ml. Concentration of MAb 2E5 required for inhibition may vary depending of the cell type studied and the concentration of soluble recombinant human TRAIL used. The neutralizing activity of MAb 2E5 has been confirmed with various sources of soluble recombinant human TRAIL including KillerTRAILTM. Method: Jurkat cells (5x104) were treated with KillerTRAILTM (300ng/ml) in the presence or absence of the MAb to TRAIL (human) (2E5) or a control IgG1 mouse monoclonal antibody. After 16 hours apoptosis was evaluated by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analyzed by flow cytometry.")
NCBI and Uniprot Product Information
Similar Products
Product Notes
The TRAIL tnfsf10 (Catalog #AAA77040) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TRAIL (human) monoclonal antibody (2E5) reacts with Human Does not cross-react with mouse TRAIL and may cross-react with other species as described in the data sheet. AAA Biotech's TRAIL can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TRAIL tnfsf10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAIL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.