FCM/FACS (Flow Cytometry)
(Flow-cytometry using the Anti-Transferrin receptor antibody R17 217.1.3 (AAA72115). RAW 264.7 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of R17 217.1.3 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rabbit anti-Mouse Transferrin receptor Monoclonal Antibody
Anti-Transferrin receptor [R17 217.1.3]
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
Transferrin receptor, Antibody; Anti-Transferrin receptor [R17 217.1.3]; CD71; Transferrin receptor protein 1; TR; ; TfR1; Trfr; anti-Transferrin receptor antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
R17 217.1.3
Specificity
The transferrin receptor is necessary for cellular iron uptake by the process of receptor-mediated endocytosis. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Transferrin receptor antibody
FCM/FACS (Flow Cytometry)
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
PMID
6319033
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the Anti-Transferrin receptor antibody R17 217.1.3 (AAA72115). RAW 264.7 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of R17 217.1.3 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the Anti-Transferrin receptor antibody R17 217.1.3 (AAA72115). RAW 264.7 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of R17 217.1.3 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of RAW 264.7 cells using anti-Transferrin receptor antibody (AAA72115) R17 217.1.3. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of R17 217.1.3 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of RAW 264.7 cells using anti-Transferrin receptor antibody (AAA72115) R17 217.1.3. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of R17 217.1.3 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-Transferrin receptor antibody
Recombinant monoclonal antibody to Transferrin receptor. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma R17 217.1.3.
The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.
The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.
Product Categories/Family for anti-Transferrin receptor antibody
References
Lesley et al, 1984. Expression of transferrin receptor on murine hematopoietic progenitors. Cell Immunol. 1984 Jan;83(1):14-25.
Note on publication: Describes the original generation of the antibody and its application to flow cytometry.
Note on publication: Describes the original generation of the antibody and its application to flow cytometry.
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The Transferrin receptor (Catalog #AAA72115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Transferrin receptor [R17 217.1.3] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Transferrin receptor can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the Transferrin receptor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transferrin receptor, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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