Application Data
(Mouse TREM2 transfected cells stained with Rat anti Mouse TREM2 followed by Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed,))
Rat TREM-2 Monoclonal Antibody | anti-TREM-2 antibody
RAT ANTI MOUSE TREM-2:Low Endotoxin
Gene Names
Trem2; TREM-2; Trem2a; Trem2b; Trem2c
Applications
Flow Cytometry, Functional Assay
Synonyms
TREM-2, Antibody; RAT ANTI MOUSE TREM-2:Low Endotoxin; anti-TREM-2 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
78.18
Form/Format
Low Endotoxin
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
249
Applicable Applications for anti-TREM-2 antibody
Flow cytometry (FC), Functional Assays (FN)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
FC: 1/25-1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
FC: 1/25-1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Mouse TREM-2 Fc fusion protein
Fusion Partners
Lymph node cells from immunised Fisher rats were fused with cells of the rat YB-2/0 myeloma cell line.
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Endotoxin
< 0.01 EU/ug
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store at -20°C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Mouse TREM2 transfected cells stained with Rat anti Mouse TREM2 followed by Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed,))
Application Data
(Mouse TREM2 transfected cells stained with Rat anti Mouse TREM2 followed by Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed,))
Application Data
(J774 cells stained with Rat anti Mouse TREM2:FITC)
Application Data
(J774 cells stained with Rat anti Mouse TREM2:FITC)
Related Product Information for anti-TREM-2 antibody
Rat anti Mouse Trem-2 antibody, clone 78.18 recognizes Triggering receptor expressed on myeloid cells 2 (TREM-2), an activating receptor of the Ig superfamily. TREM-2 forms a receptor signalling complex with DAP12 to trigger the activation of the immune response in macrophages and dendritic cells. It may be involved in chronic inflammation and may promote the production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 may also have a functional role in osteoclast formation.
Stimulation of TREM-2 with Rat anti Mouse Trem-2 antibody, clone 78.18 enhances multinucleated osteoclast formation in vitro. Clone 78.18 may also be used to block TREM-2 on pre-osteoclasts to inhibit the formation of multinuclear osteoclasts and to inhibit bone resorption by mature osteoclasts.
Stimulation of TREM-2 with Rat anti Mouse Trem-2 antibody, clone 78.18 enhances multinucleated osteoclast formation in vitro. Clone 78.18 may also be used to block TREM-2 on pre-osteoclasts to inhibit the formation of multinuclear osteoclasts and to inhibit bone resorption by mature osteoclasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
triggering receptor expressed on myeloid cells 2 isoform 2
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells 2
NCBI Official Symbol
Trem2
NCBI Official Synonym Symbols
TREM-2; Trem2a; Trem2b; Trem2c
NCBI Protein Information
triggering receptor expressed on myeloid cells 2; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2b; triggering receptor expressed on myeloid cells 2c
UniProt Protein Name
Triggering receptor expressed on myeloid cells 2
UniProt Gene Name
Trem2
UniProt Synonym Gene Names
Trem2a; Trem2b; Trem2c; TREM-2
UniProt Entry Name
TREM2_MOUSE
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Product Notes
The TREM-2 trem2 (Catalog #AAA12242) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TREM-2 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC), Functional Assays (FN). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. FC: 1/25-1/200Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the TREM-2 trem2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TREM-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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