Mouse anti-Human TREM2 Monoclonal Antibody | anti-TREM2 antibody

TREM2 antibody

Cat. Num. AAA11730

• Gene Names: TREM2; TREM-2; Trem2a; Trem2b; Trem2c
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry
• Purity: By protein-G affinity chromatography
• Synonyms: TREM2; Monoclonal Antibody; TREM2 antibody; triggering receptor expressed on myeloid cells 2 isoform 1; triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2a; TREM-2; Trem2a; Trem2b; Trem2c; anti-TREM2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG3, k
• Clone Number: 2B5
• Purity/Purification: By protein-G affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml ( determined by BCA assay) (varies by lot)
• Sequence Length: 230

• Applicable Applications for anti-TREM2 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC)
• Application Notes: WB: 1:500 ~ 1,000.; Recommended starting dilution is 1:500.; Usage: The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Immunogen: Recombinant human TREM2 (19-161aa) purified from E. coli
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

FCM (Flow Cytometry)

(Flow cytometry analysis of TREM2 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.)

IF (Immunofluorescence)

(ICC/IF analysis of TREM2 in Raw264.7 cells. The cell was stained with AAA11730 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

IF (Immunofluorescence)

(ICC/IF analysis of TREM2 in HepG2 cells. The cell was stained with AAA11730 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

SDS-PAGE

(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TREM-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : TREM2 19-161aa Transfected 293T cell lysate)

WB (Western Blot)

(Cell lysates of HeLa, MCF7, LNCaP and mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TREM2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-TREM2 antibody

The triggering receptor expressed on myeloid cells-2 (TREM2) is a membrane protein that interacts with the TYROBP protein. The TREM2 and TYROBP proteins form a complex that transmits chemical signals to activate the cell. This complex is involved in the growth and development of several types of immune cells, particularly dendritic cells and activates these cells, triggering an inflammatory response to injury or disease. And the TYROBP-TREM2 complex also activates cells in the skeletal system and in the brain and spinal cord (central nervous system).

Product Categories/Family for anti-TREM2 antibody

Immunology

References

N'Diaye EN, et al., (2009) J Cell Biol. 184 (2):215-23; Piccio L, et al., (2008) Brain. 131 (11):3081-91

NCBI and Uniprot Product Information

• NCBI GI #: 9507203
• NCBI GeneID: 54209
• NCBI Accession #: NP_061838
• NCBI GenBank Nucleotide #: NM_018965.3
• UniProt Accession #: Q9NZC2; Q8N5H8; Q8WYN6
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 2 isoform 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: TREM2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 2
• UniProt Gene Name: TREM2
• UniProt Synonym Gene Names: TREM-2