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product-image-AAA254917_FCM10.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of human TROP2 expression on MCF-7 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Mouse anti-Human TROP2 Monoclonal Antibody | anti-TACSTD2 antibody

Anti-TROP2 Antibody, Mouse Monoclonal

Gene Names
TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, ELISA
Purity
Protein A
Synonyms
TROP2, Antibody; Anti-TROP2 Antibody, Mouse Monoclonal; TROP2/TACSTD2 Antibody, Mouse MAb; tumor-associated calcium signal transducer 2; Anti-EGP-1 Antibody; Anti-EGP1 Antibody; Anti-GA733-1 Antibody; Anti-GA7331 Antibody; Anti-GP50 Antibody; Anti-M1S1 Antibody; Anti-TACD2 Antibody; Anti-TROP-2 Antibody; Anti-TROP2 Antibody; anti-TACSTD2 antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
01
Specificity
Human TROP2/TACSTD2.
No cross-reactivity in ELISA with:
Human BCAM/CD239
Human VCAM1/CD106
Human ICAM1/CD54
Human CD146/MCAM/MUC18
Human CD171/NCAML1/L1CAM
Human Cadherin-1/E-cadherin/CDH1/CD324
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TACSTD2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, ELISA
Immunogen
Recombinant Human TROP2 protein (Catalog# )
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TROP2 extracellular domain (rh TROP2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of human TROP2 expression on MCF-7 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

product-image-AAA254917_FCM10.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of human TROP2 expression on MCF-7 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

IHC (Immunohistochemisry)

(Immunochemical staining of human TROP2 in human mammary gland with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of alveolus epithelium.)

product-image-AAA254917_IHC11.jpg IHC (Immunohistochemisry) (Immunochemical staining of human TROP2 in human mammary gland with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of alveolus epithelium.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human TROP2 in human placenta with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of cytotrophoblast.)

product-image-AAA254917_IHC13.jpg IHC (Immunohiostchemistry) (Immunochemical staining of human TROP2 in human placenta with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of cytotrophoblast.)

IHC (Immunohistochemistry)

(Immunochemical staining of human TROP2 in human rectal cancer with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

product-image-AAA254917_IHC15.jpg IHC (Immunohistochemistry) (Immunochemical staining of human TROP2 in human rectal cancer with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Product Categories/Family for anti-TACSTD2 antibody
References
Ripani E, et al. (1998) Human Trop-2 is a tumor-associated calcium signal transducer. Int J Cancer. 76(5): 671-6.Wang J, et al. (2008) Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers. Mol Cancer Ther. 7(2): 280-5.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor-associated calcium signal transducer 2
NCBI Official Synonym Full Names
tumor-associated calcium signal transducer 2
NCBI Official Symbol
TACSTD2
NCBI Official Synonym Symbols
EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1
NCBI Protein Information
tumor-associated calcium signal transducer 2; epithelial glycoprotein-1; cell surface glycoprotein TROP2; cell surface glycoprotein Trop-2; pancreatic carcinoma marker protein GA7331; pancreatic carcinoma marker protein GA733-1; gastrointestinal tumor-ass
UniProt Protein Name
Tumor-associated calcium signal transducer 2
UniProt Gene Name
TACSTD2
UniProt Synonym Gene Names
GA733-1; M1S1; TROP2
UniProt Entry Name
TACD2_HUMAN

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Product Notes

The TACSTD2 tacstd2 (Catalog #AAA254917) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-TROP2 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TROP2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, ELISA. Researchers should empirically determine the suitability of the TACSTD2 tacstd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TROP2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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