Mouse anti-Human TSC22D3 / GILZ Monoclonal Antibody | anti-TSC22D3 antibody

Mouse Monoclonal [clone 3A5] (IgG1,k) to Human TSC22D3 / GILZ

Cat. Num. AAA51924

• Gene Names: TSC22D3; DIP; GILZ; hDIP; DSIPI; TSC-22R
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified from ascites by Protein A
• Synonyms: TSC22D3 / GILZ, Antibody; Mouse Monoclonal [clone 3A5] (IgG1,k) to Human TSC22D3 / GILZ; Anti-TSC22D3 / GILZ Antibody (clone 3A5) IHC-plus; TSC22D3; DSIPI; DIP; DSIP-immunoreactive peptide; GILZ; TSC-22 related protein; Protein DIP; TSC22 domain family protein 3; HDIP; TSC-22-like protein; TSC-22-related protein; TSC-22R; TSC22 domain family; member 3; Human TSC22D3; anti-TSC22D3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3A5
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Concentration: 0.34 mg/ml (varies by lot)
• Sequence Length: 134

• Applicable Applications for anti-TSC22D3 antibody: Activity Assay, IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: TSC22D3 (NP_932174, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Species: TSC22D3/GILZ antibody was raised againts Human
• Immunogen: TSC22D3 / GILZ antibody was raised against tSC22D3 (NP_932174, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

ELISA

(Detection limit for recombinant GST tagged TSC22D3 is approximately 0.03 ng/ml as a capture antibody.)

WB (Western Blot)

(Western blot of TSC22D3 expression in transfected 293T cell line. Lane 1: Transfected Cell Lysate Lane 2: Non-transfected cell lysate.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to TSC22D3 on HeLa cell (antibody concentration 10 ug/ml).)

IHC (Immunohiostchemistry)

(Anti-TSC22D3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry)

(Anti-TSC22D3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-TSC22D3 antibody

The DSIPI protein has homology to porcine DIP (DSIP-immunoreactive peptide). The DSIPI protein contains a highly conserved leucine zipper region and belongs to the TSC-22/DIP/BUN family of transcriptional regulators. DSIPI is postulated to also function as a transcriptional regulator.

NCBI and Uniprot Product Information

• NCBI GI #: 37622901
• NCBI GeneID: 1831
• NCBI Accession #: NP_004080.2
• NCBI GenBank Nucleotide #: NM_004089.3
• UniProt Accession #: Q99576; Q5H9S3; Q5JRI9; Q6FIH6; Q8NAI1; Q8WVB9; Q9UBN5; Q9UG13
• Molecular Weight: 13,286 Da
• NCBI Official Full Name: TSC22 domain family protein 3 isoform 2
• NCBI Official Synonym Full Names: TSC22 domain family, member 3
• NCBI Official Symbol: TSC22D3
• NCBI Official Synonym Symbols: DIP; GILZ; hDIP; DSIPI; TSC-22R
• NCBI Protein Information: TSC22 domain family protein 3; TSC-22-like protein; TSC-22 related protein; TSC-22-related protein; DSIP-immunoreactive peptide; DSIP-immunoreactive leucine zipper protein; delta sleep-inducing peptide immunoreactor; delta sleep inducing peptide, immunoreactor; glucocorticoid-induced leucine zipper protein
• UniProt Protein Name: TSC22 domain family protein 3
• UniProt Gene Name: TSC22D3
• UniProt Synonym Gene Names: DSIPI; GILZ; Protein DIP; hDIP; GILZ; TSC-22R
• UniProt Entry Name: T22D3_HUMAN