Mouse anti-Rat Tubulin, beta III Monoclonal Antibody | anti-TUBB3 antibody

Anti-Beta III Tubulin

Cat. Num. AAA72750

• Reactivity: Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G purified (Protein G purified culture supernatant.)
• Synonyms: Tubulin, beta III, Antibody; Anti-Beta III Tubulin; anti-TUBB3 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: AA10
• Specificity: Specific for the ~55kDa beta III tubulin protein in Western Blot of rat brain extracts.
• Purity/Purification: Protein G purified (Protein G purified culture supernatant.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots
• Sequence Length: 450

• Applicable Applications for anti-TUBB3 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Antigen: Synthetic peptide corresponding to beta III tubulin.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues specific to beta III tubulin conjugated to KLH
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in rat. It is expected that this antibody will have wide species cross reactivity.
• Biological Significance: Tubulin is the major constituent of microtubules, existing as a heterodimer of the alpha and beta subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)
• Preparation and Storage: For long term storage-80 degree C is recommended, but shorter term storage at -20 degree C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 degree C.

Application Data

Related Product Information for anti-TUBB3 antibody

Purified mouse monoclonal antibody

References

• Lee MK, Rebhun LI, Frankfurter A (1990) Posttranslational modification of class III beta tubulin. Proc Natl Acad Sci USA 87(18): 7195-7199.
• Katsetos CD, Herman MM, Mork SJ (2003) Class III beta tubulin in human development and cancer. Cell Motil Cytoskeleton 55(2):77-96.

NCBI and Uniprot Product Information

• NCBI GI #: 145966774
• NCBI GeneID: 246118
• NCBI Accession #: NP_640347.2
• NCBI GenBank Nucleotide #: NM_139254.2
• UniProt Accession #: Q4QRB4; Q8K5B6
• NCBI Official Full Name: tubulin beta-3 chain
• NCBI Official Synonym Full Names: tubulin, beta 3 class III
• NCBI Official Symbol: Tubb3
• NCBI Protein Information: tubulin beta-3 chain; neuron-specific class III beta-tubulin
• UniProt Protein Name: Tubulin beta-3 chain
• UniProt Gene Name: Tubb3
• UniProt Entry Name: TBB3_RAT