IF (Immunofluorescence)
(ICC/IF analysis of TXNRD1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of TXNRD1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human TXNRD1 Monoclonal Antibody | anti-TXNRD1 antibody
TXNRD1 antibody
Gene Names
TXNRD1; TR; TR1; TXNR; TRXR1; GRIM-12
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
TXNRD1, Antibody; TXNRD1 antibody; Thioredoxin reductase 1; GRIM-12; MGC9145; TR; TR1; TRXR1; TXNR; Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein; GRIM 12; GRIM12; KDRF; KM 102 derived reductase like factor; Oxidoreductase; Thioredoxin reductase 1 cytoplasmic; Thioredoxin reductase GRIM 12; Thioredoxin reductase TR1; TR 1; TRXR 1; TXNRD 1.; anti-TXNRD1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT30F1
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
649
Applicable Applications for anti-TXNRD1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human TXNRD1 (161-647aa) purified from E. coli
Clone Note
Anti-human TXNRD1 mAb, clone AT30F1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TXNRD1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of TXNRD1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of TXNRD1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of TXNRD1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of TXNRD1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TXNRD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate Lane 3.: PC3 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TXNRD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate Lane 3.: PC3 cell lysate)
Related Product Information for anti-TXNRD1 antibody
TXNRD1, also known as Thioredoxin reductase 1, is a part of a selenium-containing pyridine nucleotidedisulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. Inhibition of TXNRD1 activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases.
Product Categories/Family for anti-TXNRD1 antibody
References
Ma X., et al. (2002) J Biol Chem. 277 (25): 22460-8. ; Javvadi P (2010) Cancer Res. 70 (5): 1941-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,873 Da
NCBI Official Full Name
thioredoxin reductase 1, cytoplasmic isoform 5
NCBI Official Synonym Full Names
thioredoxin reductase 1
NCBI Official Symbol
TXNRD1
NCBI Official Synonym Symbols
TR; TR1; TXNR; TRXR1; GRIM-12
NCBI Protein Information
thioredoxin reductase 1, cytoplasmic
UniProt Protein Name
Thioredoxin reductase 1, cytoplasmic
UniProt Gene Name
TXNRD1
UniProt Synonym Gene Names
GRIM12; KDRF; TR; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein
Similar Products
Product Notes
The TXNRD1 txnrd1 (Catalog #AAA48264) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TXNRD1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TXNRD1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TXNRD1 txnrd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TXNRD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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