Mouse anti-Human Uroplakin 1A Monoclonal Antibody | anti-UPK1A antibody

Uroplakin 1A (Urothelial Differentiation Marker)

Cat. Num. AAA214888

• Gene Names: UPK1A; UP1A; UPIA; UPKA; TSPAN21
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Uroplakin 1A, Antibody; Uroplakin 1A (Urothelial Differentiation Marker); UP1A; UPIA; UPKA; TSPAN21; anti-UPK1A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: UPK1A/2922
• Specificity: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 273

• Applicable Applications for anti-UPK1A antibody: IHC (Immunohistochemistry), ELISA
• Positive Control: Urinary Bladder.
• Immunogen: Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 19q13.12
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2922) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2922). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Bladder stained with Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2922).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin 1A Mouse Monoclonal Antibody (UPK1A/2922).)

References

Sun TT, et al. Adv Exp Med Biol. 1999;462:7-18; discussion 103-14.

NCBI and Uniprot Product Information

• NCBI GI #: 527498307
• NCBI GeneID: 11045
• NCBI Accession #: NP_001268372.1
• NCBI GenBank Nucleotide #: NP_001268372.1
• UniProt Accession #: O00322
• Molecular Weight: 29kDa
• NCBI Official Full Name: uroplakin-1a isoform 2
• NCBI Official Synonym Full Names: uroplakin 1A
• NCBI Official Symbol: UPK1A
• NCBI Official Synonym Symbols: UP1A; UPIA; UPKA; TSPAN21
• NCBI Protein Information: uroplakin-1a
• UniProt Protein Name: Uroplakin-1a
• UniProt Gene Name: UPK1A
• UniProt Synonym Gene Names: TSPAN21; UP1a; Tspan-21; UPIa; UPKa
• UniProt Entry Name: UPK1A_HUMAN