FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA278988 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
USP24 recombinant antibody
USP24 Recombinant Monoclonal Antibody
Gene Names
USP24; FLJ31309; KIAA1057
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Affinity purified
Synonyms
USP24, Recombinant Antibody; USP24 Recombinant Monoclonal Antibody; Ubiquitin carboxyl-terminal hydrolase 24 (EC 3.4.19.12)(Deubiquitinating enzyme 24)(Ubiquitin thioesterase 24)(Ubiquitin-specific-processing protease 24); USP24; KIAA1057; USP24 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
11E3
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for USP24 recombinant antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human USP24
Conjugation
Non-conjugated
Research Area
Epigenetics and Nuclear Signaling; Cell biology
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA278988 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA278988 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cell with AAA278988 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 569-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cell with AAA278988 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 569-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Related Product Information for USP24 recombinant antibody
Ubiquitin-specific protease that regulates cell survival in various contexts through modulating the protein stability of some of its substrates including DDB2, MCL1 or TP53. Plays a positive role on ferritinophagy where ferritin is degraded in lysosomes and releases free iron. {ECO:0000269|PubMed:23159851, ECO:0000269|PubMed:29695420}.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
294,365 Da
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 24
NCBI Official Synonym Full Names
ubiquitin specific peptidase 24
NCBI Official Symbol
USP24
NCBI Official Synonym Symbols
FLJ31309; KIAA1057
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 24; OTTHUMP00000009832; ubiquitin thioesterase 24; deubiquitinating enzyme 24; ubiquitin thiolesterase 24; ubiquitin specific protease 24; ubiquitin-specific processing protease 24; ubiquitin-specific-processing prote
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 24
UniProt Gene Name
USP24
UniProt Synonym Gene Names
KIAA1057
UniProt Entry Name
UBP24_HUMAN
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Product Notes
The USP24 usp24 (Catalog #AAA278988) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The USP24 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP24 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the USP24 usp24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP24, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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