IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of CHO-V5 cells using 3 ug Rabbit anti V5-Tag antibody (AAA283343). Western blot was performed from the immunoprecipitate using Rabbit anti V5-Tag antibody (AAA283343) at a dilution of 1:5000.)
Rabbit V5-Tag Monoclonal Antibody | anti-V5 antibody
Rabbit anti V5-Tag mAb
Reactivity
Species independent
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Affinity purification
Synonyms
V5-Tag, Antibody; Rabbit anti V5-Tag mAb; V5;V5 tag;V5-tag; anti-V5 antibody
Host
Rabbit
Reactivity
Species independent
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
GKPIPNPLLGLDST
Applicable Applications for anti-V5 antibody
ELISA, IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to V5-Tag.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of CHO-V5 cells using 3 ug Rabbit anti V5-Tag antibody (AAA283343). Western blot was performed from the immunoprecipitate using Rabbit anti V5-Tag antibody (AAA283343) at a dilution of 1:5000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of CHO-V5 cells using 3 ug Rabbit anti V5-Tag antibody (AAA283343). Western blot was performed from the immunoprecipitate using Rabbit anti V5-Tag antibody (AAA283343) at a dilution of 1:5000.)
WB (Western Blot)
(Western blot analysis of lysates from 293T cells, using Rabbit anti V5-Tag mAb (AAA283343) at 1:10000-1:320000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of lysates from 293T cells, using Rabbit anti V5-Tag mAb (AAA283343) at 1:10000-1:320000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with V5 Protein, using Rabbit anti V5-Tag mAb (AAA283343) at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with V5 Protein, using Rabbit anti V5-Tag mAb (AAA283343) at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Related Product Information for anti-V5 antibody
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Product Categories/Family for anti-V5 antibody
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Product Notes
The V5 (Catalog #AAA283343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti V5-Tag mAb reacts with Species independent and may cross-react with other species as described in the data sheet. AAA Biotech's V5-Tag can be used in a range of immunoassay formats including, but not limited to, ELISA, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the V5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GKPIPNPLLG LDST. It is sometimes possible for the material contained within the vial of "V5-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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