Application Data
(Fig.2: SDS-PAGE analysis of purified BEP-5D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BEP-5D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent 3.)
Rat VEGFR2 Monoclonal Antibody | anti-VEGFR2 antibody
Rat anti-human VEGFR2 monoclonal antibody
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Applications
ELISA, ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
VEGFR2, Antibody; Rat anti-human VEGFR2 monoclonal antibody; anti-VEGFR2 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a/kappa
Clone Number
BEP-5D8
Specificity
anti-human/pig VEGFR2 (epitope on the third extracellular immunoglobulin-like domain)
Purity/Purification
Protein G
Sequence Length
1356
Applicable Applications for anti-VEGFR2 antibody
ELISA, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
Phosphate buffered saline, pH 7.2
Immunogen
Genetic immunisation with cDNA encoding human VEGFR2
Selection
Based on recognition of the complete native protein expressed on transfected mammalian cells
Preparation and Storage
Short term: 2 degree C - 8 degree C
Long term: -20 degree C (avoid repeated freezing and thawing)
Long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(Fig.2: SDS-PAGE analysis of purified BEP-5D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BEP-5D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent 3.)
Application Data
(Fig.2: SDS-PAGE analysis of purified BEP-5D8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified BEP-5D8 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent 3.)
FCM/FACS (Flow Cytometry)
(Fig.1: GM-1007. BOSC23 cells were transiently transfected with an expression vector encoding either human VEGFR2 (green curve), pig VEGFR2 (blue curve) or an irrelevant protein (control transfectant, red curve). Binding of BEP-5D8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human and pig VEGFR2 transfected cells.)
FCM/FACS (Flow Cytometry)
(Fig.1: GM-1007. BOSC23 cells were transiently transfected with an expression vector encoding either human VEGFR2 (green curve), pig VEGFR2 (blue curve) or an irrelevant protein (control transfectant, red curve). Binding of BEP-5D8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with human and pig VEGFR2 transfected cells.)
Related Product Information for anti-VEGFR2 antibody
The vascular endothelial growth factor receptor-2 (VEGFR-2) plays an important role in the control of angiogenesis. This molecule is expressed on the surface of circulating endothelial stem cells as well as on adult vascular endothelial cells and other cell types (1). It is a 200-kDa type I transmembrane protein, consisting of a 19 amino acid leader peptide, a 744 amino acid extracellular domain made up of 7 immunoglobulin-like domains, a 24 amino acid transmembrane region and a 568 amino acid cytoplasmic domain. Interactions with VEGF ligands will stimulate angiogenesis, required in wound healing but also misused by tumours to promote their vascularization (2). The current monoclonal antibody was developed by genetic immunization against the complete extracellular domain of human VEGFR-2 and has been shown to be specific for the native target molecule and its counterpart in pigs using FACscan analyses on viable, transiently-transfected human BOSC23 cells (see Fig. 1). It binds to an epitope on the third extracellular immunoglobulin-like domain.
References
1. Paz K, Zhu Z. (2005) Development of angiogenesis inhibitors to vascular endothelial growth factor receptor 2. Current status and future perspective. Front. Biosci. 10:1415-1439.
2. Youssoufian H., Hicklin D.J., Rowinsky E.K. (2007) Monoclonal antibodies to the vascular endothelial growth factor receptor-2 in cancer therapy. Clin. Cancer Res. 13 (18 Suppl): 5544- 5528.
2. Youssoufian H., Hicklin D.J., Rowinsky E.K. (2007) Monoclonal antibodies to the vascular endothelial growth factor receptor-2 in cancer therapy. Clin. Cancer Res. 13 (18 Suppl): 5544- 5528.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,634 Da
NCBI Official Full Name
vascular endothelial growth factor receptor 2
NCBI Official Synonym Full Names
kinase insert domain receptor
NCBI Official Symbol
KDR
NCBI Official Synonym Symbols
FLK1; CD309; VEGFR; VEGFR2
NCBI Protein Information
vascular endothelial growth factor receptor 2
UniProt Protein Name
Vascular endothelial growth factor receptor 2
UniProt Gene Name
KDR
UniProt Synonym Gene Names
FLK1; VEGFR2; VEGFR-2; FLK-1; KDR
UniProt Entry Name
VGFR2_HUMAN
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Product Notes
The VEGFR2 kdr (Catalog #AAA71495) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's VEGFR2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the VEGFR2 kdr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VEGFR2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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