WB (Western Blot)
(Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.)
Mouse VGLUT1 Monoclonal Antibody | anti-Slc17a7 antibody
VGLUT1 Antibody: FITC
Gene Names
Slc17a7; BNPI; Vglut1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Synonyms
VGLUT1, Antibody; VGLUT1 Antibody: FITC; BNPI; SLC17A1; SLC17A1/VGLUT1; Solute Carrier family 17 member 7; Vesicular glutamate transporter 1; VGLUT 1; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; anti-Slc17a7 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S28-9
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
560
Applicable Applications for anti-Slc17a7 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Conjugate
FITC
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide.
Preparation and Storage
-20 degree C
WB (Western Blot)
(Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.)
WB (Western Blot)
(Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X. Courtesy of: Leilei Wang, Ph.D. UT Southwestern Medical Center at Dallas.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X. Courtesy of: Leilei Wang, Ph.D. UT Southwestern Medical Center at Dallas.)
Related Product Information for anti-Slc17a7 antibody
Background Info: Detects ~52kDa. No cross-reactivity against VGLUT2.
Scientific Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
Scientific Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
Product Categories/Family for anti-Slc17a7 antibody
References
1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163. 2. Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,333 Da
NCBI Official Full Name
vesicular glutamate transporter 1
NCBI Official Synonym Full Names
solute carrier family 17 (vesicular glutamate transporter), member 7
NCBI Official Symbol
Slc17a7
NCBI Official Synonym Symbols
BNPI; Vglut1
NCBI Protein Information
vesicular glutamate transporter 1; solute carrier family 17 member 7; brain-specific Na-dependent inorganic phosphate cotransporter; brain-specific Na(+)-dependent inorganic phosphate cotransporter; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
UniProt Protein Name
Vesicular glutamate transporter 1
UniProt Gene Name
Slc17a7
UniProt Synonym Gene Names
Bnpi; Vglut1; VGluT1
UniProt Entry Name
VGLU1_RAT
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Product Notes
The Slc17a7 slc17a7 (Catalog #AAA103180) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VGLUT1 Antibody: FITC reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VGLUT1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the Slc17a7 slc17a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VGLUT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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