Mouse Villin Monoclonal Antibody | anti-VIL1 antibody

Villin (GI-Mucosal & Urogenital Brush Border Marker)

Cat. Num. AAA214554

• Gene Names: VIL1; VIL; D2S1471
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: Villin, Antibody; Villin (GI-Mucosal & Urogenital Brush Border Marker); VIL1; Villin-1; Villin1; anti-VIL1 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: VIL1/1325
• Specificity: Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 827

• Applicable Applications for anti-VIL1 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant human Villin fragment of 133 amino acid residues (aa179-311)
• Cellular Localization: Cytoplasmic & Cell Surface
• Hu-chromosome Location: 2q35
• Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

WB (Western Blot)

(Western Blot of A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1325).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Colon stained with Villin Monoclonal Antibody (VIL1/1325).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Rectum stained with Villin Monoclonal Antibody (VIL1/1325).)

References

West BA, Isaac CA, Carboni JM, Morrow JS, Mooseker MS, Barwick KW. Localization of villin, a cytoskeletal protein specific to microvilli, in human ileum and colon and in colonic neoplasms. Gastroenterology. 1988; 94:343-52. Bacchi CE, Gown AM. Distribution and pattern of expression of villin, a gastrointestinal-associated cytoskeletal protein, in human carcinomas: a study employing paraffin-embedded tissue. Lab Invest. 1991; 64:418-24.

NCBI and Uniprot Product Information

• NCBI GI #: 194394237
• NCBI GeneID: 7429
• NCBI Accession #: NP_009058.2
• NCBI GenBank Nucleotide #: NM_007127.2
• UniProt Accession #: P09327; Q53S11; Q96AC8; B2R9A7
• Molecular Weight: 93kDa
• NCBI Official Full Name: villin-1
• NCBI Official Synonym Full Names: villin 1
• NCBI Official Symbol: VIL1
• NCBI Official Synonym Symbols: VIL; D2S1471
• NCBI Protein Information: villin-1
• UniProt Protein Name: Villin-1
• UniProt Gene Name: VIL1
• UniProt Synonym Gene Names: VIL